在pH 7.4的生理条件下, 以溴化乙锭(EB)作为荧光探针, 利用荧光光谱、紫外-可见吸收光谱、共振散射光谱法结合盐效应和DNA熔点(Tm)实验研究了柯里拉京(Cor)与小牛胸腺DNA分子之间的相互作用机制。实验结果表明, Cor静态猝灭DNA-EB体系的荧光。Cor与DNA作用后, 其特征吸收峰强度发生减色效应; 与DNA作用导致Cor在480.5 nm处的共振散射峰增强, 并在330.2 nm处出现新共振散射峰。盐效应对Cor与DNA分子相互作用的影响较小。与Cor作用引起DNA的Tm值升高5.5 ℃。由此推断, Cor与DNA相互作用的主要方式为嵌插, 两者间形成了超分子体系。通过计算获得Cor与DNA间结合常数(KA)为5.82×103 L/mol (298 K)、2.47×104 L/mol (310 K), 它们之间的作用为熵驱动的自发、吸热过程, 疏水作用力是主要的非共价作用方式。

Under the physiological condition of pH 7.4, using ethidium bromide (EB) as the fluorescent probe, the interaction mechanism between coriolis (Cor) and calf thymus DNA was studied by UV-Vis absorption spectroscopy, resonance scattering spectroscopy combined with salt effect and DNA melting point. Cor can quench the fluorescence of EB-DNA system through a static process. The subtractive effect of the characteristic absorption peak occurrs after Cor interacts with DNA. The interaction leads to the enhancement of Cor resonance at 481 nm and a new resonance scattering peak at 330 nm. The salt effect has a little influence on the interaction between Cor and DNA. The interaction leads to the increase of the Tm of DNA by 5.5 ℃. It is deduced that the main way of the interaction between Cor and DNA is intercalation, and a supramolecular system is formed between them. The binding constant (KA) between Cor and DNA is 5.82×103 L/mol (298 K) and 2.47×104 L/mol (310 K). The interaction between Cor and DNA is entropy-driven spontaneous, endothermic. Hydrophobic forces are the main non-covalent mode of action.