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Principle and Experiment of Protein Detection Based on Optical Fiber Sensing

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Abstract

A method for detecting protein molecules based on the tilted fiber Bragg grating (TFBG) surface plasma resonance (SPR) is proposed to achieve the quick online real-time detection of trace amount of proteins. The detection principles of the TFBG-SPR protein molecular probe are analyzed, and its feasibility is demonstrated. The intermediary material between the protein molecules and the golden layer outside of the fiber gratings is cysteamine hydrochloride. When the concentration of the cysteamine hydrochloride solution is 2 M, the shift of the TFBG resonance peak is 2.23 nm, illustrating that the cysteamine hydrochloride modifies the gold film successfully. IgG antigen solution is poured on the surface of the cysteamine hydrochloride modifying the gold-deposited TFBG. Finally, antigen-antibody hybridization experiment is carried out with a 10 mg/mL antibody solution, and after two hours of hybridization the resonance peak of the TFBG shifts 5.1 nm, which validates the feasibility and effectiveness of the TFBG-SPR protein molecular probe.

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DOI:10.1007/s13320-017-0383-7

所属栏目:Regular

基金项目:This work was financially supported by the National Nature Science Foundation of China (Nos. 61271073 and 61473175) and was supported by the Fundamental Research Funds of Shandong University (No. 2015JC040).

收稿日期:2016-09-18

修改稿日期:2017-06-12

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Qi JIANG:School of Control Science and Engineering, Shandong University, Jinan, 250061, China
Mengwei XUE:School of Control Science and Engineering, Shandong University, Jinan, 250061, China
Pei LIANG:School of Control Science and Engineering, Shandong University, Jinan, 250061, China
Chengjia ZHANG:State Key Lab of Microbial Technology, School of Life Science, Shandong University, Jinan, 250100, China
Jianqiang LIN:State Key Lab of Microbial Technology, School of Life Science, Shandong University, Jinan, 250100, China
Jun OUYANG:School of Materials Science and Engineering, Shandong University, Jinan, 250061, China

联系人作者:Qi JIANG(xuemengweistu@163.com)

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引用该论文

Qi JIANG,Mengwei XUE,Pei LIANG,Chengjia ZHANG,Jianqiang LIN,Jun OUYANG. Principle and Experiment of Protein Detection Based on Optical Fiber Sensing[J]. Photonic Sensors, 2017, 7(4): 317-324

Qi JIANG,Mengwei XUE,Pei LIANG,Chengjia ZHANG,Jianqiang LIN,Jun OUYANG. Principle and Experiment of Protein Detection Based on Optical Fiber Sensing[J]. Photonic Sensors, 2017, 7(4): 317-324

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