激光生物学报, 2018, 27 (1): 53, 网络出版: 2018-04-23
大豆Lea5基因过表达提高烟草抗旱和耐盐性的研究
Overexpression of Soybean Lea5 Gene Confers Resistance to Drought and Tolerance to Salinity in Tobacoo
大豆Lea5基因 转基因植物 干旱抗性 耐盐性 Soybean Lea5 gene transgenic plant RT-PCR RT-PCR resistance to drought tolerance to salinity
摘要
大豆Lea5基因是LEA基因家族成员之一。为分析大豆Lea5基因的功能, 构建了大豆Lea5基因植物过表达载体pBI121-Lea5。通过农杆菌介导法转化烟草,获得TL-06、TL-09、TL-17和TL-32等4个转大豆Lea5基因烟草株系。RT-PCR分析表明大豆Lea5基因在4个转基因烟草株系的T2代植株均有不同程度的表达。以转基因TL-09和TL-32株系T2代植株为材料, 进行了干旱和盐渍处理, 结果表明, 2个转大豆Lea5基因烟草株系T2代植株对干旱和盐渍的抗性显著强于野生型烟草, 说明大豆Lea5基因可以提高植物抗干旱和盐碱的能力。
Abstract
In order to investigate biological function of soybean Lea5 gene, one of LEA gene family, the overexpression vector of pBI121-Lea5 was constructed. Through leaf discs transformation by Agrobacterium (LB4404: pBI121-Lea5), four transgenic plants of TL-06, TL-09, TL-17 and TL-32 were selected and identified. Analysis by RT-PCR shows that soybean Lea5 gene was expressed in T2 generation plants of the four transgenic lines in different level. Treated with drought and salinity, T2 generation plants of TL-09 and TL-32 displayed more resistance to drought and more tolerance to salinity. This indicates that overexpression of soybean Lea5 gene confers resistance to drought and tolerance to salinity in plant.
何道一, 夏仁杰, 周秀杰, 徐萍莉. 大豆Lea5基因过表达提高烟草抗旱和耐盐性的研究[J]. 激光生物学报, 2018, 27(1): 53. HE Daoyi, XIA Renjie, ZHOU Xiujie, XU Pingli. Overexpression of Soybean Lea5 Gene Confers Resistance to Drought and Tolerance to Salinity in Tobacoo[J]. Acta Laser Biology Sinica, 2018, 27(1): 53.
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