激光与光电子学进展, 2018, 55 (9): 090005, 网络出版: 2018-09-08   

拉曼光谱检测中荧光抑制方法及其应用分析 下载: 2251次

Fluorescence Suppression Methods in Raman Spectroscopy Detection and Their Application Analysis
作者单位
中国计量大学光学与电子科技学院, 浙江 杭州 310018
摘要
在拉曼光谱分析中, 由于样品及污染物等常会产生强的荧光信号, 严重影响样品拉曼光谱信号的检测, 从而限制了该项技术的应用。文中综述了各种解决荧光干扰问题方法的原理、实现方式、性能特点, 对几种常见的荧光抑制方法(荧光淬灭法、光漂白法、紫外/红外光激发法、移频激发法、小波变换法)在不同领域的应用进行具体分析, 包括激发波长、荧光来源、荧光信号的变化、荧光抑制效果、对拉曼信号的影响等。不仅展现出每种方法的优势, 也说明了其局限性。
Abstract
In the analysis of Raman spectroscopy, the sample and contaminants often produce strong fluorescence signal which seriously affects the detection of Raman spectrum signal produced by the sample, and limits the application of the technology. In recent years, a number of methods have been developed to solve the problem of fluorescence interference. In this paper, the fundamental principle, implementation and performance characteristics of various methods were reviewed and summarized, and the application of several common fluorescence suppression methods(fluorescence quenching method, light bleaching method, ultraviolet light excitation method, infrared light excitation method, shifted-excitation Raman difference spectroscopy and wavelet transform)were also concretely analyzed in different fields including excitation wavelength, fluorescence source, variation of fluorescence signal, the effect of fluorescence suppression, the influence of the Raman signal and so on. It not only shows the advantages of each method, but also illustrates its disadvantages.

朱磊磊, 冯爱明, 金尚忠, 徐冰冰, 刘开元, 王杰. 拉曼光谱检测中荧光抑制方法及其应用分析[J]. 激光与光电子学进展, 2018, 55(9): 090005. Zhu Leilei, Feng Aiming, Jin Shangzhong, Xu Bingbing, Liu Kaiyuan, Wang Jie. Fluorescence Suppression Methods in Raman Spectroscopy Detection and Their Application Analysis[J]. Laser & Optoelectronics Progress, 2018, 55(9): 090005.

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