表面增强拉曼光谱(surface-enhanced Raman scattering,SERS)能够有效解决常规拉曼中信号极弱问题,在低浓度分析物的痕量检测甚至单分子的检测中具有重要的应用前景,是化学、生物、环境等领域重要的分析手段。在SERS中,高性能SERS基底的实现是关键。本文以微球自组装技术为基础,制备了一种大面积、廉价、高效的SERS基底并对其进行了形貌表征和拉曼增强光谱研究。通过开展R6G分子的SERS研究发现,此种SERS基底对R6G拉曼散射信号的增强倍数是一般粗糙基底的五倍以上。结合数值模拟分析和系统的实验研究,得到了微球直径、纳米颗粒的高度等参数对基底表面附近局域热点和SERS增强倍数的影响规律,给出了最优化的SERS基底参数。本文工作可为SERS研究提供高性能的SERS基底。

Surface-enhanced Raman scattering(SERS) can pave the way for the trace detection of low-concentration analyses,even single molecular detection,and owns greatly potential application prospect in chemistry,biology,environment,and so on.Development of high-performance SERS substrate is one of the most important research topics in SERS technology.In this work,we achieve the fabrication,characterization,and SERS effect research of a kind of high-performance,low-cost,and large area SERS substrate.The Raman scattering intensity from R6G molecule can be effectively enhanced by this SERS substrate about five times compared with that with roughness Au film.We carried out systematic numerical simulation and experiment research on the influence of micro-sphere diameter and nano-particle height on the Raman scattering enhanced factor,and find an optimized structure parameters.Our work will provide a kind of high-performance SERS substrate.