基于所制备的高品质法布里-珀罗(F-P)光学微腔, 研究了一种基于激光信号的腔内高灵敏度熔解(HRM)曲线检测方法, 即将F-P光学微腔作为微激光腔, 将嵌入式饱和染料作为增益介质, 以产生的激光信号作为检测信号, 通过温度扫描, 实现了对碱基错配DNA分子的高灵敏度检测与筛查。分别研究了25个碱基对和50个碱基对的目标DNA及碱基错配DNA的熔解曲线, 理论及实验结果表明：基于激光信号的HRM检测技术具有低的熔解温度和高的信噪比。

We study a high-resolution melting (HRM) analysis method based on laser signal in self-made Fabry-Perot (FP) optical microcavity with high quality. We utilize the F-P optical microcavity as the micro-laser cavity, intercalated saturation dyes as the gain media and emitted laser signal as the detection signal to achieve high-resolution detection and screening of mismatched DNA by temperature scanning. We investigate on the melting curves of target DNA and mismatched DNA with 25 base-pairs and 50 base-pairs, respectively. Theoretical and experimental results indicate that the HRM detection method based on laser signal has lower melting temperature and higher signal-to-noise ratio.