本文通过溶胶-凝胶法制备了Fe-TiO2和Ni-TiO2纳米颗粒, 并研究这两种纳米颗粒体外光动力疗法(PDT)对HL60细胞的灭活效果。通过透射电镜(TEM)、能谱仪(EDS)、X射线衍射(XRD)、紫外-可见光(UV-Vis)吸收光谱等方法对纳米颗粒进行表征。使用CCK-8法分别测定Fe-TiO2和Ni-TiO2对HL60细胞的灭活效果。结果表明, 不同终值浓度及掺杂量的Fe-TiO2和Ni-TiO2纳米颗粒对HL60细胞的暗毒性较低, 但是PDT效率均显著高于未掺杂的TiO2。在各自的最佳作用参数下, PDT灭活效率分别达到72.5%±1.6%和56.4%±1.2%。此外, 还对这两种纳米颗粒灭活效果的差异进行了探讨。

In this paper, Fe-TiO2 and Ni-TiO2 nanoparticles were synthesized by sol-gel method. The inactivation of HL60 cells in vitro photodynamic therapy was studied. The nanoparticles were characterized by transmission electron microscope (TEM), energy dispersive spectrometer(EDS), X-ray diffraction (XRD), ultraviolet-visible (UV-Vis) absorption spectrum etc. The inactivation of HL60 cells by Fe-TiO2 and Ni-TiO2 was analyzed by CCK-8 assay. The experimental results show that Fe-TiO2 and Ni-TiO2 nanoparticles with different final concentration and doping amount have lower dark toxicity to HL60 cells, but the PDT efficiency is significantly higher than that of pure TiO2. Under the optimal parameters, the PDT inactivation efficiency reached 72.5%±1.6% and 56.4%±1.2% respectively. In addition, the differences in the inactivation effects of these two nanoparticles were also discussed.