自适应共焦检眼镜以其高分辨率、动态成像等光学特性，已经在生物医学和临床医学的多个领域得到了广泛而具体的应用。为了能够将非圆形光瞳滤波器等瞳面调制技术运用于其中，并不对波前探测产生影响，系统需要利用两个光源分别进行成像和波前校正。本文首先设计了一套基于双光源的自适应共焦检眼镜，对不同光源的人眼像差进行测量，分析了其主要差异。然后对双光源系统的像差校正能力和高分辨成像能力进行了验证，系统闭环后的图像的亮度、对比度和分辨率都有了显著的提高。最后验证了使用半圆形光瞳实现暗场成像的可行性，并得到了模拟人眼的明暗场图像。

Adaptive confocal laser ophthalmoscope with the high-resolution and dynamic imaging ability has been widely applied in specific biomedical and clinical medical fields. In order to apply the noncircular pupil filter and other pupil modulation technology without influence in wavefront detection, the system needs two light sources for imaging and aberration correction respectively. This paper has designed an adaptive optics scanning laser ophthalmoscopy with two sources, and then analyzed the differences of the aberration of the two light sources. Then, the aberration correction and high-resolution imaging ability of the system have been verified，and the brightness, contrast and resolution of the image have been significantly improved after close-loop. Finally, we have studied the feasibility of realizing the dark field imaging by semi-circular pupil and obtained the bright and dark field images of the artificial eye.