半导体光电, 2019, 40 (1): 20, 网络出版: 2019-03-25  

对NpHR光敏感蛋白具有刺激作用的光电极研制

Study on Monochromatic Optrode Used for Stimulating Photo-Sensitive Protein NpHR
作者单位
中山大学 电子与信息工程学院 光电材料与技术国家重点实验室, 广州 510006
摘要
光电极是研究光遗传学不可或缺的工具之一。 波长为460nm的蓝光可以对ChR2神经蛋白产生刺激, 而波长为580nm的黄光可以刺激NpHR神经蛋白, 对生物体的反应产生抑制。目前, 蓝光波段的光电极具有很高的功率密度, 可以满足光遗传学的研究, 而黄光波段半导体发光器件的发光效率较低, 因此对黄光波段的光电极研究较少。在蓝宝石衬底蓝光光电极研制的基础上, 通过激发黄色荧光粉和量子点两种形式获得黄光, 并通过沉积SiO2/TiO2分布式布拉格反射镜(DBR)有效滤除了蓝光波段的激发光, 最后通过沉积Ag金属反射镜增强黄光信号, 制备出荧光粉基和量子点基黄光光电极。与荧光粉基黄光光电极相比, 量子点基黄光光电极具有更高的功率密度、更窄的光谱半宽和更薄的厚度。在1~10mA的注入电流下, 量子点基黄光光电极的功率密度为4.46~15.37mW/mm2, 满足刺激NpHR神经蛋白的要求。
Abstract
As an essential tool for the research of optogenetics, the optrode can be used to stimulate photo-sensitive proteins like ChR2 and NpHR, which are sensitive to the light with the emission peak of 460 and 580nm, respectively. The optical electrodes in the blue light band have a high power density, which can satisfy the research of optogenetics. However, the luminous efficiency of semiconductor LEDs in the yellow light band is low, thus few study was performed on the optical electrodes in yellow band. In this paper, the study of blue light optrode based on the sapphire substrate was performed, and yellow light was generated by the two forms of stimulating yellow phosphor and quantum dots; exciting light at blue band was effectively removed by depositing SiO2/TiO2 distributed Bragg reflector (DBR), yellow light signals were enhanced by depositing Ag metal reflector, thus the yellow light electrodes were fabricated based on phosphors and quantum dots. Compared with yellow light electrodes based on phosphors, the QD-based electrodes own much higher power density, narrower spectral half-width and thinner thickness. The power density of QD-based yellow light electrode is 4.46~15.37mW/mm2 under 1~10mA injection current, which can meet the requirements of stimulating NpHR neuroprotein.

温耀辉, 侯雅倩, 梁捷智, 张佰君. 对NpHR光敏感蛋白具有刺激作用的光电极研制[J]. 半导体光电, 2019, 40(1): 20. WEN Yaohui, HOU Yaqian, LIANG Jiezhi, ZHANG Baijun. Study on Monochromatic Optrode Used for Stimulating Photo-Sensitive Protein NpHR[J]. Semiconductor Optoelectronics, 2019, 40(1): 20.

关于本站 Cookie 的使用提示

中国光学期刊网使用基于 cookie 的技术来更好地为您提供各项服务,点击此处了解我们的隐私策略。 如您需继续使用本网站,请您授权我们使用本地 cookie 来保存部分信息。
全站搜索
您最值得信赖的光电行业旗舰网络服务平台!