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高清折反式视频显微镜

High definition catadioptric video microscope

高丰   李琦   向阳   宁旭  
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摘要

为了实现对细胞工厂中细胞的实时监测, 设计一款高分辨率、长工作距离的视频显微镜。采用卡塞格林式物镜与折射式显微系统相结合的设计方法, 在反射式系统中加入厚弯月形透镜补偿系统产生的像差, 缩短光学系统的横向尺寸, 校正像差。针对被观察细胞的具体特征, 设计了一种工作距离100mm、分辨率1.2μm、线视场2mm的视频显微镜, 达到高清水平, 系统很好地校正色差、球差、场曲和畸变, 达到了平场消色差显微镜的要求。实现对细胞状态的高清实时监测。

Abstract

In order to realize the real-time monitoring of cells in the cell factory, a video microscope with high resolution and long working distance is designed. A design method is used by combining the Cassegrain objective lens with the refractive microscope system, adding a thick meniscus lens to compensate the aberration caused by reflective system, shortening the lateral size of the optical system and correcting the aberration. In connection with specific characteristics for observed cells, a video microscope with a working distance of 100mm, a resolution of 1.2μm, and a line field of view of 2mm is designed. The system reaches high definition level. The system excellently corrects chromatic aberration, spherical aberration, field curvature and distortion, which achieves the requirements of the flat field achromatic microscope and realized high definition real-time monitoring of cell status.

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中图分类号:TB133

所属栏目:光学设计

基金项目:吉林省科技厅重大科技专项(20160203009YY)

收稿日期:2018-05-28

修改稿日期:2018-08-28

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作者单位    点击查看

高丰:长春理工大学 光电工程学院, 吉林 长春 130022
李琦:长春理工大学 光电工程学院, 吉林 长春 130022
向阳:长春理工大学 光电工程学院, 吉林 长春 130022
宁旭:长春理工大学 光电工程学院, 吉林 长春 130022

联系人作者:高丰(1321396119@qq.com)

备注:高丰(1994-), 男 , 硕士研究生, 从事光电仪器的开发与研究。

【1】胡德敬, 陈玉萍, 谢嘉祥. 用显微镜简捷测量液体折射率的浓度[J]. 光学精密工程,1999,7(01):117-120.
Hu Dengjing, Chen Yuping, Xie Jiaxiang. Simple measurement of the refractive index of a liquid with a microscope[J]. Optical and Precision Engineering,1999,7(01):117-120.

【2】文里云, 迪米特. 一种大数值孔径、长工作距离的显微镜物镜[P]. 江苏:CN205844614U,2016-12.28.
Wu Liyun, Di Mite. A large numerical aperture, long working distance microscope objective[P]. Jiangsu:CN205844614U,2016-12.28.

【3】Pacheco Shaun, Wang Chengliang, Chawla Monica K, et al. High resolution, high speed, long working distance, large field of view confocal fluorescence microscope[J]. Scientific reports,2017,7(1):1-10.

【4】嵇晓强, 程揭章, 李琦, 等. 细胞工厂光学显微成像与图像处理技术[J]. 光子学报,2015,44(07):150-157.
Ji Xiaoqiang, Cheng Jiezhang, Li Qi, et al. Cell factory optical microscope imaging and image processing technology[J]. Optics Journal, 2015,44(07):150-157.

【5】高健, 向阳, 尹志东, 等. 一种长工作距视频显微镜光学系统设计[J]. 光学仪器,2014,36(05):416-419.
Gao Jian, Xiang Yang, Yin Zhidong, et al. The optical system of long working distance video microscope[J]. Optical Instruments,2014,36(05):416-419.

【6】薛金来, 巩岩, 李佃蒙. N.A.0.75平场复消色差显微物镜光学设计[J]. 中国光学,2015,8(06):957-963.
Xue Jinlai, Gong Yan, Li Dianmeng. Optical design of N.A.0.75 plan apochromatic microscope objectives[J]. China Optics,2015,8(06):957-963.

【7】Sebastian Stach, Wiktoria Sapota, Zygmunt Wróbel, et al. Assessment of possibilities of ceramic biomaterial fracture surface reconstruction using laser confocal microscopy and long working distance objective lenses[J]. Microscopy Research and Technique,2016,79(5):385-392.

【8】王培芳, 向阳, 王继凯, 等. 光电显微镜的光学系统设计[J]. 长春理工大学学报:自然科学版,2015,38(05):17-21.
Wang Peifang, Xiang Yang, Wang Jikai, et al. The optical system design of photoelectric microscope[J]. Journal of Changchun University of Science and Technology:Natural Science Edition,2015,38(05):17-21.

【9】梅培俊, 许键. 适用显微镜质量测试的高分辨率测量镜头设计[J]. 光学仪器,2017,39(05):64-69.
Mei Peijun, Xu Jian. High resolution measurement lens design for microscope quality testing[J]. Optical Instruments,2017,39(05):64-69.

【10】杨国锋. 光学系统中心偏误差分析方法研究[D]. 西安工业大学,2015.
Yang Guofeng. Research on central error analysis method of optical system[D]. XiAn Industrial University,2015.

【11】李灿, 宋淑梅, 刘英, 等. 折反式眼底相机光学系统设计[J]. 光学精密工程,2012,20(08):1710-1717.
Li Can, Song Shumei, Liu Ying. Catadioptric camera optical system design[J]. Optical and Precision Engineering, 2012,20(08):1710-1717.

【12】Infante F, Perdu P, Lewis D. Magnetic microscopy for 3D devices: Defect localization with high resolution and long working distance on complex system in package[J]. Microelectronics Reliability,2009,49(9):1169-1174.

【13】李婕, 明景谦, 卢若飞. 一种改进型的红外卡塞格林光学系统设计[J]. 红外技术,2010,32(02):76-80.
Li Jie, Ming Jingqian, Lu Rufei. Design of an ameliorating infrared Cassegrain optical system[J]. Infrared Technology, 2010, 32(02):76-80.

【14】郭贵新, 贾宗合, 付秀华, 等. 基于散粒磨料振动抛光非球面加工技术研究[J]. 应用光学,2017,38(01):89-93.
Guo Guixin, Jia Zonghe, Fu Xiuhua, et al. Research on aspherical surface processing technology based on vibration abrasive polishing[J]. Applied Optics,2017,38(01):89-93.

【15】Yasuko Terada, Hirokatsu Yumoto, Akihisa Takeuchi, et al. New X-ray microprobe system for trace heavy element analysis using ultraprecise X-ray mirror optics of long working distance[J]. Nuclear Inst. and Methods in Physics Research A,2009,616(2):270-272.

【16】王彬, 蒋世磊. 卡塞格林系统计算机辅助装调技术研究[J]. 光学仪器,2008,30(01):50-54.
Wang Bin, Jiang Shilei. Study on computer-aided alignment method of Cassegrain system[J]. Optical Instruments,2008,30 (01):50-54.

【17】刘磊, 李景林, 吕清涛, 等. 大口径反射光学系统装调装置设计研究[J]. 光学精密工程,2005,13(S1):134-137.
Liu Lei, Li Jing Lin, Lv Jingtao, et al. Research on the design of large diameter reflective optical system mounting device[J]. Optical and Precision Engineering,2005,13(S1):134-137.

【18】卢政伟. 消除中心遮拦的激光扩束器研究[D]. 中国科学院长春光学精密机械与物理研究所,2017.
Lv Zhengwei. Study on laser beam expander for eliminating center blocking[D]. Changchun Institute of Optics, Fine Mechanics and Physics, Chinese Academy of Sciences, 2017.

引用该论文

GAO Feng,LI Qi,XIANG Yang,NING Xu. High definition catadioptric video microscope[J]. Optical Technique, 2019, 45(2): 136-141

高丰,李琦,向阳,宁旭. 高清折反式视频显微镜[J]. 光学技术, 2019, 45(2): 136-141

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