为了实现对细胞工厂中细胞的实时监测, 设计一款高分辨率、长工作距离的视频显微镜。采用卡塞格林式物镜与折射式显微系统相结合的设计方法, 在反射式系统中加入厚弯月形透镜补偿系统产生的像差, 缩短光学系统的横向尺寸, 校正像差。针对被观察细胞的具体特征, 设计了一种工作距离100mm、分辨率1.2μm、线视场2mm的视频显微镜, 达到高清水平, 系统很好地校正色差、球差、场曲和畸变, 达到了平场消色差显微镜的要求。实现对细胞状态的高清实时监测。

In order to realize the real-time monitoring of cells in the cell factory, a video microscope with high resolution and long working distance is designed. A design method is used by combining the Cassegrain objective lens with the refractive microscope system, adding a thick meniscus lens to compensate the aberration caused by reflective system, shortening the lateral size of the optical system and correcting the aberration. In connection with specific characteristics for observed cells, a video microscope with a working distance of 100mm, a resolution of 1.2μm, and a line field of view of 2mm is designed. The system reaches high definition level. The system excellently corrects chromatic aberration, spherical aberration, field curvature and distortion, which achieves the requirements of the flat field achromatic microscope and realized high definition real-time monitoring of cell status.