利用自组装的数字全息表面等离子体共振成像技术,分别检测了两种具有不同分子质量的桃胶多糖(PGP-1与PGP-2)与半乳糖凝集素-3的相互作用。制备了表面等离子体共振成像生物芯片,同时检测了具有不同浓度的桃胶多糖样品与半乳糖凝集素-3的结合过程,制作了标准曲线,并计算了相互作用的结合平衡常数。结果表明,两种具有不同分子质量的桃胶多糖可以直接结合半乳糖凝集素-3,其中PGP-1的结合平衡常数为8.36×10 ⁵ M ^-,PGP-2的结合平衡常数为1.24×10 ⁵ M ^-。结合曲线符合生物分子相互作用的规律,证明了该方法在多通量生物检测中的可行性。该方法实验装置简单、易操作、无需标记、成本低,在高通量分析技术中具有一定的应用前景。

The interactions between galectin-3 and two types of peach-gum polysaccharides with different molecular weights (PGP-1 and PGP-2) were detected herein via self-assembly surface plasma resonance (SPR) imaging based on digital holography. Different concentrations of peach-gum polysaccharides and Galectin-3 were simultaneously detected on an SPR biochip prepared for detecting the concentrations. The standard curves were derived and the binding equilibrium constants of the reactions were calculated. The results show that the two types of peach-gum polysaccharides can directly bind to Galectin-3. The binding equilibrium constants of PGP-1 and PGP-2 are 8.36×10 5 and 1.24×10 5 M -, respectively. The binding curves conform to the law of biomolecular interaction, demonstrating the feasibility of the proposed method in high-throughput biological detection. The proposed method can be easily controlled and is simple, label-free, and inexpensive. It is potentially applicable to the high-throughput microanalysis technology.