光学 精密工程, 2019, 27 (6): 1270, 网络出版: 2019-07-29   

皮肤反射式共聚焦显微成像自适应图像亮度调节

Image brightness adaptive adjustment during skin imaging by reflectance confocal microscopy
缪新 1,2张运海 1,2黄维 2
作者单位
1 中国科学技术大学, 安徽 合肥 230026
2 中国科学院 苏州生物医学工程技术研究所 江苏省医用光学重点实验室, 江苏 苏州 215163
摘要
在皮肤反射式共聚焦显微成像过程中, 为了实现图像亮度的快速调节, 提出了一种图像亮度自适应调节方法。通过实验建立光强控制电压与图像亮度之间的关系模型, 划分图像极端亮度区间与适度亮度区间, 采用分段调节策略, 将初始图像从极端亮度区间快速调整至适度亮度区间, 在适度亮度区间内通过线性补偿调节至目标灰度均值。对不同深度、不同位置的皮肤组织进行实时成像, 图像初始亮度存在着过亮、过暗和适中等各种情况, 上述亮度自适应调节方法均能实现快速亮度调节, 调节迭代次数为2~3, 调节后图像灰度均值达到最优值70左右。实验结果表明, 这种自适应图像亮度调节方法快速、有效, 能够满足皮肤共聚焦成像检测的需要。
Abstract
For quick adjustment of image brightness during skin imaging with reflectance confocal microscopy, an adaptive brightness adjustment method was proposed. The association between laser power control voltage and image brightness was established by experiments. Images can have two types of brightness: extreme and moderate. Using segmental adjustment, brightness of the initial image could be quickly changed from extreme to moderate. Finally, the optimal brightness was obtained using linear compensation. Real-time imaging of skin tissues at different depths and locations can show if the initial image is too bright, too dark, or of moderate brightness. Subsequently, quick adjustment of the brightness can be achieved. The number of iterations is 2-3, and the optimal image brightness is approximately 70. The results show that this method is fast and can effectively meet the needs of skin imaging with reflectance confocal microscopy.

缪新, 张运海, 黄维. 皮肤反射式共聚焦显微成像自适应图像亮度调节[J]. 光学 精密工程, 2019, 27(6): 1270. MIAO Xin, ZHANG Yun-hai, HUANG Wei. Image brightness adaptive adjustment during skin imaging by reflectance confocal microscopy[J]. Optics and Precision Engineering, 2019, 27(6): 1270.

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