光谱学与光谱分析, 2019, 39 (9): 2713, 网络出版: 2019-09-28  

混合载气高场不对称波形离子迁移谱中He-N2比例对挥发性有机物混叠峰分离的影响研究

Study on the Effect of He-N2 Ratio in Mixed Carrier Gas on Separation of VOC Aliasing Peaks in FAIMS
作者单位
1 合肥工业大学汽车与交通工程学院, 安徽 合肥 230009
2 中国科学院合肥智能机械研究所传感技术国家重点实验室, 安徽 合肥 230031
3 中国科学技术大学自动化系, 安徽 合肥 230027
摘要
载气混合是提高高场不对称波形离子迁移谱(FAIMS)离子分离能力的一个重要方法, 在生物大分子质谱研究领域已得到广泛应用, 但缺乏在环境小分子领域的研究。 选取挥发性有机物(VOCs)中芳香烃、 醇、 烷烃、 酸、 酮类中的五种物质(邻二甲苯、 异丁醇、 正己烷、 乙酸、 丙酮)为研究对象, 研究了混合气体中He-N2比例对VOCs中单体-二聚体离子混叠峰峰位置、 分离度及总离子通过率的影响。 实验结果表明, 随着FAIMS载气中He比例的增加, 5种VOCs中单体及二聚体离子峰峰位置发生偏移, 且单体峰与二聚体峰偏移程度不同, 单体峰偏移量先增加后减少, 而二聚体峰峰位置偏移量逐渐增加; 随着FAIMS载气中He比例的增加, 五种VOCs离子混叠峰的分离度逐渐增加并趋于饱和, 邻二甲苯、 异丁醇、 正己烷、 乙酸、 丙酮5种样品的混叠峰分离度达到饱和时对应He比例分别是: 20%, 30%, 10%, 40%和20%; 随着FAIMS载气中He比例的增加, 邻二甲苯、 异丁醇、 正己烷、 丙酮的离子信号强度无明显变化, 乙酸的离子信号强度下降明显。 该研究为提高FAIMS对环境小分子的分离能力提供了一种可行的方法。 同时, 也验证了高电场下布朗定律在小分子离子应用上的有效性。
Abstract
Carrier gas mixing as one of the most important methods to improve the separation ability of high Field Asymmetric waveform Ion Mobility Spectrometry (FAIMS) has been widely used in the field of bio macromolecule mass spectrometry, while there is a lack of some research in environmental small molecules. In this paper, five typical volatile organic compounds (VOCs), including o-xylene, isobutanol, n-hexane, acetic acid and acetone, were chosen to study the effect of N2-He mixing ratio on the peak position, resolution and ion pass rate of monomers and dimer ion. The results showed that with the increase of the proportion of He in the carrier gas of FAIMS, the peak position of the monomer and dimer ion in five VOCs shifts, and the peak of the monomer and the dimer were different, and the monomer peak shift increased first and then decreased, while the dimer peak position shift increased gradually. With the increase of the percentage of He, the resolution of FAIMS for aliasing peaks of five VOCs gradually increased and tended to saturate at last, where the saturated helium ratio was: 20%, 30%, 10%, 40% and 20%, respectively. In addition, with the increase of the percentage of helium, the signal intensity of o-xylene, isobutanol, n-hexane and acetone had no obvious change, while that of acetic acid decreased significantly. This study provided a feasible method for improving the separation ability of FAIMS and validating the effectiveness of Blanc’s law under high electric field applied in the field of small molecules.

石海峡, 徐青, 王晗, 刘友江, 李山, 胡俊, 李跃, 陈池来. 混合载气高场不对称波形离子迁移谱中He-N2比例对挥发性有机物混叠峰分离的影响研究[J]. 光谱学与光谱分析, 2019, 39(9): 2713. SHI Hai-xia, XU Qing, WANG Han, LIU You-jiang, LI Shan, HU Jun, LI Yue, CHEN Chi-lai. Study on the Effect of He-N2 Ratio in Mixed Carrier Gas on Separation of VOC Aliasing Peaks in FAIMS[J]. Spectroscopy and Spectral Analysis, 2019, 39(9): 2713.

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