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Fluorescence lifetime imaging microscopy and its applications in skin cancer diagnosis

Fluorescence lifetime imaging microscopy and its applications in skin cancer diagnosis

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Abstract

Fluorescence lifetime (FLT) of fluorophores is sensitive to the changes in their surrounding microenvironment, and hence it can quantitatively reveal the physiological characterization of the tissue under investigation. Fluorescence lifetime imaging microscopy (FLIM) provides not only morphological but also functional information of the tissue by producing spatially resolved image of fluorophore lifetime, which can be used as a signature of disorder and/or malignancy in diseased tissues. In this paper, we begin by introducing the basic principle and common detection methods of FLIM. Then the recent advances in the FLIM-based diagnosis of three different skin cancers, including basal cell carcinoma (BCC), squamous cell carcinoma (SCC) and malignant melanoma (MM) are reviewed. Furthermore, the potential advantages of FLIM in skin cancer diagnosis and the challenges that may be faced in the future are prospected.

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DOI:10.1142/s1793545819300040

基金项目:This work has been partially supported by The 111 Project (B17035), Open Research Fund Program of the State Key Laboratory of Low Dimensional Quantum Physics (KF201713), State Key Laboratory of Transient Optics and Photonics, Chinese Academy of Sciences (SKLST201804) and the Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province (GD201711).

收稿日期:2019-03-26

修改稿日期:2019-06-10

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Lixin Liu:School of Physics and Optoelectronic Engineering, Xidian University, Xi'an 710071, P. R. China
Qianqian Yang:School of Physics and Optoelectronic Engineering, Xidian University, Xi'an 710071, P. R. China
Meiling Zhang:School of Physics and Optoelectronic Engineering, Xidian University, Xi'an 710071, P. R. China
Zhaoqing Wu:School of Physics and Optoelectronic Engineering, Xidian University, Xi'an 710071, P. R. China
Ping Xue:State Key Laboratory of Low-Dimensional Quantum Physics, Department of Physics, Tsinghua University, Beijing 100084, P. R. China

联系人作者:Lixin Liu(lxliu@xidian.edu.cn)

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引用该论文

Lixin Liu,Qianqian Yang,Meiling Zhang,Zhaoqing Wu,Ping Xue. Fluorescence lifetime imaging microscopy and its applications in skin cancer diagnosis[J]. Journal of Innovative Optical Health Sciences, 2019, 12(5): 1930004

Lixin Liu,Qianqian Yang,Meiling Zhang,Zhaoqing Wu,Ping Xue. Fluorescence lifetime imaging microscopy and its applications in skin cancer diagnosis[J]. Journal of Innovative Optical Health Sciences, 2019, 12(5): 1930004

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