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Implementation and application of FRET-FLIM technology

Implementation and application of FRET-FLIM technology

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Abstract

With the development of the new detection methods and the function of fluorescent molecule, researchers hope to further explore the internal mechanisms of organisms, monitor changes in the intracellular microenvironment, and dynamic processes of molecular interactions in cells. Fluorescence resonance energy transfer (FRET) describes the energy transfer process between donor fluorescent molecules and acceptor fluorescent molecules. It is an important means to detect protein-protein interactions and protein conformation changes in cells. Fluorescence lifetime imaging microscopy (FLIM) enables noninvasive measurement of the fluorescence lifetime of fluorescent particles in vivo. The FRET-FLIM technology, which is use FLIM to quantify and analyze FRET, enables real-time monitoring of dynamic changes of proteins in biological cells and analysis of protein interaction mechanisms. The distance between donor and acceptor and their respective fluorescent lifetime, which are of great importance for studying the mechanism of intracellular activity can be obtained by data analysis and algorithm fitting.

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DOI:10.1142/s1793545819300106

基金项目:This work has been partially supported by the National Natural Science Foundation of China (61722508/61525503/61620106016/81727804). The National Key Research and Development Program of China (2017YFA0700402), Guangdong Natural Science Foundation Innovation Team (2014A030312008), and Shenzhen Basic Research Project (JCYJ20150930104948169/JCYJ201603281 44746940/GJHZ20160226202139185/JCYJ20170412 105003520).

收稿日期:2019-03-14

修改稿日期:2019-07-11

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Shiqi Wang:Key Laboratory of Optoelectronic Devices and Systems of Guangdong Province, College of Physics and Optoelectronic Engineering, Shenzhen University, Shenzhen, 518060, P. R. China
Binlin Shen:Key Laboratory of Optoelectronic Devices and Systems of Guangdong Province, College of Physics and Optoelectronic Engineering, Shenzhen University, Shenzhen, 518060, P. R. China
Sheng Ren:Key Laboratory of Optoelectronic Devices and Systems of Guangdong Province, College of Physics and Optoelectronic Engineering, Shenzhen University, Shenzhen, 518060, P. R. China
Yihua Zhao:Key Laboratory of Optoelectronic Devices and Systems of Guangdong Province, College of Physics and Optoelectronic Engineering, Shenzhen University, Shenzhen, 518060, P. R. China
Silu Zhang:Key Laboratory of Optoelectronic Devices and Systems of Guangdong Province, College of Physics and Optoelectronic Engineering, Shenzhen University, Shenzhen, 518060, P. R. China
Junle Qu:Key Laboratory of Optoelectronic Devices and Systems of Guangdong Province, College of Physics and Optoelectronic Engineering, Shenzhen University, Shenzhen, 518060, P. R. China
Liwei Liu:Key Laboratory of Optoelectronic Devices and Systems of Guangdong Province, College of Physics and Optoelectronic Engineering, Shenzhen University, Shenzhen, 518060, P. R. China

联系人作者:Liwei Liu(liulw@szu.edu.cn)

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引用该论文

Shiqi Wang,Binlin Shen,Sheng Ren,Yihua Zhao,Silu Zhang,Junle Qu,Liwei Liu. Implementation and application of FRET-FLIM technology[J]. Journal of Innovative Optical Health Sciences, 2019, 12(5): 1930010

Shiqi Wang,Binlin Shen,Sheng Ren,Yihua Zhao,Silu Zhang,Junle Qu,Liwei Liu. Implementation and application of FRET-FLIM technology[J]. Journal of Innovative Optical Health Sciences, 2019, 12(5): 1930010

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