为了探究羟基磷灰石(HAP)纳米颗粒与硒化镉量子点(CdSe QD)的共轭物HAP-QD作为生物纳米温度探针的可能性,将CdSe QD与HAP纳米颗粒进行化学偶联,得到HAP-QD共轭物,并研究了HAP-QD荧光谱的温度特性。首先用硅烷偶联剂KH550对HAP表面进行氨基修饰;然后在偶联活化剂的作用下将表面修饰有羧基的CdSe QD与表面修饰有氨基的HAP进行共价偶联,得到HAP-QD共轭物;最后测量了298~318 K温度范围内CdSe QD和HAP-QD的荧光谱。实验结果表明,HAP-QD的荧光谱峰位随温度的升高会出现红移,且具有良好的线性关系。

In order to study the possibility of the HAP-QD conjugate of hydroxyapatite (HAP) nanoparticles and cadmium selenide quantum dots (CdSe QD) as a biological nano-temperature probe, HAP nanoparticles are chemically coupled with CdSe QD to obtain HAP-QD conjugate, and further study the temperature characteristics of HAP-QD fluorescence spectrum. First, the surface of HAP is modified by silane coupling agent KH550. Then, under the action of coupling activator, the CdSe QD with a carboxyl group modified on the surface and HAP with an amino group modified on the surface are covalently coupled to obtain a HAP-QD conjugate. Finally, the fluorescence spectra of CdSe QD and HAP-QD are measured at the temperature range of 298--318 K. The experimental results show that the fluorescence peak position of HAP-QD demonstrates a redshift and has a good linear relationship with the increase of temperature.