光学学报, 2019, 39 (12): 1217002, 网络出版: 2019-12-06
苯酚胁迫下藻类光合活性参数的响应规律 下载: 993次
Response of Photosynthetic Activity Parameters of Algae Under Phenol Stress
生物光学 苯酚 蛋白核小球藻 光合活性参数 急性毒性 biotechnology phenol chlorella pyrenoidosa photosynthetic activity parameters acute toxicity
摘要
以蛋白核小球藻为受试对象,以藻类光合活性参数为毒性评价指标,研究了在苯酚急性毒性作用下的72 h与70 min内,不同光合活性参数的响应规律。结果显示:苯酚对光合活性参数Fv/Fm、rP、α、Ek、JVPⅡ的抑制效应显著;在苯酚急性毒性的作用下,光合活性参数的毒性响应可在短时间内达到稳定,5 min可作为苯酚毒性分析的响应时间;Fv/Fm、rP、Ek、JVP Ⅱ对苯酚毒性具有良好的剂量响应关系,不同光合活性参数对苯酚的毒性响应程度不同,0.2~1.2 g/L的苯酚对Fv/Fm、rP、Ek、JVP Ⅱ的抑制率分别为-3%~19%,9%~67%,15%~74%,17%~37%。
Abstract
Chlorella pyrenoidosa is used as the subject and the photosynthetic activity parameters of this type of algaes are used as a toxicity evaluation index. The responses of different photosynthetic activity parameters to the acute toxicity of phenol are studied over 72 h and 70 min. The results show that phenol has significant inhibitory effects on the photosynthetic activity parameters Fv/Fm, rP, α, Ek, and JVP II. Further, under the acute toxicity of phenol, the toxic responses of the aforementioned photosynthetic activity parameters are stable over a short time, where 5 min can be used as a response time for the toxicity analysis of phenol. Finally, the photosynthetic parameters Fv/Fm, rP, Ek, and JVP II show a good dose-response relationship to phenol toxicity and each parameter responds differently to the toxicity of phenol. Across a concentration range of 0.2-1.2 g/L, the inhibition rates of Fv/Fm, rP, Ek, and JVP II are -3%-19%, 9%-67%, 15%-74%, and 17%-37%, respectively.
陈敏, 殷高方, 赵南京, 甘婷婷, 王翔, 华卉, 冯春, 刘建国. 苯酚胁迫下藻类光合活性参数的响应规律[J]. 光学学报, 2019, 39(12): 1217002. Min Chen, Gaofang Yin, Nanjing Zhao, Tingting Gan, Xiang Wang, Hui Hua, Chun Feng, Jianguo Liu. Response of Photosynthetic Activity Parameters of Algae Under Phenol Stress[J]. Acta Optica Sinica, 2019, 39(12): 1217002.