在显微成像中,基于光强传输方程的双相机动态相位成像是定量观测活细胞运动的一种有效方法。但是相机安装带来的误差使得两个相机的视场之间存在一定差异,导致利用光强传输方程求解获取的相位不准确。为此,提出了一种基于棋盘格标定的双相机图像校正方法,以消除相机间视场的不匹配问题,校正后的匹配精度可达到亚像素级,大大提高了相位成像的正确率。首先对标准微透镜阵列进行定量成像测量,验证所提方法的准确性和可行性,再对游动的雨生红球藻细胞进行动态相位成像,成像结果表明该方法在动态生物成像领域具有一定的应用前景。

In microscopic imaging, dual-camera dynamic phase imaging based on the transport of intensity equation (TIE) is an effective method for the quantitative observation of phase imaging of living cells. However, camera installation errors cause the difference in field-of-views of two cameras. Consequently, the phase obtained using TIE is inaccurate. This study proposes a correction method based on checkerboard calibration to eliminate the mismatch with the sub-pixel aligning accuracy. The feasibility and accuracy of the proposed method are verified by quantitatively imaging measurement for a standard microlens array. The dynamic phase imaging of the living Haematococcus pluvialis cells is successfully realized, indicating that the method has the potential for wide applications in the field of dynamic bio-microscopy imaging.