光学学报, 2019, 39 (10): 1011002, 网络出版: 2019-10-09
光场显微成像微尺度流场三维重建方法研究 下载: 1666次
Three-Dimensional Reconstruction of Micro-Scale Flow Field Based on Light Field Microscopic Imaging
图像处理 三维显微粒子测速技术 微尺度流动 光场显微成像 反卷积重建 image processing three-dimensional micro-particle velocimetry micro-scale flow light field microscopic imaging deconvolution reconstruction
摘要
现有三维显微粒子图像测速系统在获取示踪粒子空间位置时需要扫描或多视角成像,导致系统复杂,无法实现微尺度流动瞬态三维速度场测量等问题,为此,提出一种基于微透镜阵列的光场显微粒子图像测速技术。该技术利用单个相机一次曝光即可获取微流场中示踪粒子的瞬时光场信息,进一步结合基于波动光学理论的光场显微成像的点扩展函数模型,用反卷积算法重建出微尺度流场中示踪粒子的瞬时空间位置分布。分析讨论了重建分辨率和空间位置误差,并开展了微尺度流场重建实验研究,验证了光场显微成像微尺度流场重建方法的可行性。
Abstract
The existing three-dimensional micro-particle image velocimetry systems obtain the spatial position of tracer particles through scanning or imaging from multiple perspectives, which leads to the complexity of the system and difficulty in making an instantaneous measurement of three-dimensional velocity in micro-scale flow. In this paper, a light field micro-particle image velocimetry technique based on a microlens array is proposed. In this technique, the instantaneous light field information of tracer particles in the micro-scale flow field can be recorded by a single camera in a single photographic exposure. Further, in combination with the point spread function model of the light field microscopic imaging system calculated by wave optics theory, the instantaneous spatial position distribution of tracer particles in the micro-scale flow field can be reconstructed by a deconvolution method. The reconstruction resolution and the spatial position error are analyzed and discussed. Experiments on micro-scale flow field reconstruction are carried out, and the feasibility of the reconstruction method for light field microscopic imaging is verified.
宋祥磊, 李舒, 顾梦涛, 张彪, 许传龙. 光场显微成像微尺度流场三维重建方法研究[J]. 光学学报, 2019, 39(10): 1011002. Xianglei Song, Shu Li, Mengtao Gu, Biao Zhang, Chuanlong Xu. Three-Dimensional Reconstruction of Micro-Scale Flow Field Based on Light Field Microscopic Imaging[J]. Acta Optica Sinica, 2019, 39(10): 1011002.