光学 精密工程, 2017, 25 (3): 584, 网络出版: 2017-04-18   

便携式上转换荧光试纸条检测仪的研制

Development of portable up-conversion photoluminescence strip detector
作者单位
1 河北工业大学 电气工程学院, 天津 300401
2 天津科技大学 食品工程与生物技术学院, 天津 300457
3 天津大学 精密仪器与光电子工程学院, 天津 300072
摘要
为了实现对上转换荧光试纸条的定量检测, 研制了便携式上转换荧光试纸条检测仪, 并通过实际测试验证了仪器的主要性能。首先, 介绍了上转换荧光技术以及上转换荧光试纸条的测试原理, 描述了以光学扫描检测系统和电路系统为核心的便携式荧光试纸条检测仪的设计方案, 能够将试纸条的荧光信号转变为电信号并进行处理; 然后, 基于设计方案研制了检测仪样机, 并对实际检测试纸条采集到的信号进行优化; 最后, 对检测仪的性能进行了测试。测试结果表明: 仪器稳定、重复性好, 样本检测标准差小于0.004; 线性响应特性良好, 拟合出的浓度标准曲线决定系数为0.996 3。该检测仪结构精巧、功耗低、性能良好, 很好地满足了上转换荧光试纸条定量检测的需求。
Abstract
A portable up-conversion fluorescent strip detector was developed to achieve the quantitative analysis of the up-conversion fluorescent strip. The performance of the detector was verified by practical measurements. The up-conversion fluorescent technology and the principle of up-conversion fluorescent strip were introduced firstly. The up-conversion fluorescent strip detector contained two critical components that were optical scanning system and electrical system, enabling the conversion of fluorescence signals to electric signals. Then, the prototype of the detector was developed and tested experimentally, therein the sampled signals detected by the strip were optimized. This apparatus proves great stability and repeatability, and the standard deviation of the detection is less than 0.004. The linear response characteristic is also excellent as the coefficient of determination of concentration standard curve reaches 0.996 3. This detector has a low power consumption and high performance with an exquisite structure, thus satisfying the requirements of quantitative analysis through up-conversion fluorescent strip.
参考文献

[1] ZHOU J, LIU Z, LI F Y. Upconversion nanophosphors for small-animal imaging [J]. Chemical Society Reviews, 2012, 41(3): 1323-1349.

[2] GUAN Y L, QU S N, LI B, et al.. Ratiometric fluorescent nanosensors for selective detecting cysteine with upconversion luminescence [J]. Biosensors & Bioelectronics, 2016, 77: 124-130.

[3] WANG F, LIU X G. Recent advances in the chemistry of lanthanide-doped upconversion nanocrystals [J].Chemical Society Reviews, 2009, 38(4): 976-989.

[4] HUA F, ZHANG P P, ZHANG F L, et al.. Development and evaluation of an up-converting phosphor technology-based lateral flow assay for rapid detection of Francisella tularensis [J]. Scientific Reports, 2015, 5: 17178.

[5] NGOM B, GUO Y C, WANG X L, et al.. Development and application of lateral flow test strip technology for detection of infectious agents and chemical contaminants: a review [J]. Analytical and Bioanalytical Chemistry, 2010, 397 (3): 1113-1135.

[6] YAN C L, DADVAND A, ROSEI F, et al.. Near-IR photoresponse in new up-converting CdSe/NaYF4: Yb, Er nanoheterostructures [J]. Journal of the American Chemical Society, 2010, 132(26): 8868-8869.

[7] 李春凤, 赵勇, 王晓英, 等. 基于上转发光免疫层析技术的常见食源性致病菌快速检测方法研究与评价[J].军事医学, 2015, 39(2): 128-132.

    LI CH F, ZHAO Y, WANG X Y, et al.. Development of up-converting phosphor technology based lateral flow assay for quantitative detection of foodborne pathogens [J]. Military Medical Sciences, 2015, 39(2): 128-132. (in Chinese)

[8] 李向丽, 谭贵良, 张娜, 等. 上转换发光纳米技术及其在食品安全检测中应用研究进展[J].现代食品科技, 2014, 30(8): 280-287.

    LI X L, TAN G L, ZHANG N, et al.. Upconversion fluorescent nanoparticles technology in food inspection [J]. Modern Food Science and Technology, 2014, 30(8): 280-287. (in Chinese)

[9] 卢健, 周蕾, 赵永凯, 等. 上转换发光免疫试纸条扫描检测系统研究[J].光子学报, 2006, 35(4): 555-560.

    LU J, ZHOU L, ZHAO Y K, et al.. Scanning detection system of UPL-based immunoassay test strip [J]. Acta Photonica Sinica, 2006, 35(4): 555-560. (in Chinese)

[10] KETTLE J, CHANG S W, HORIE M. IR sensor based on low bandgap organic photodiode with up-converting phosphor [J]. IEEE Sensors Journal, 2015, 15(6): 3221-3224.

[11] 田青, 罗金平, 刘晓红, 等. 生物发光法细菌快速检测仪的研制及应用[J].光学 精密工程, 2010, 18(4): 771-778.

    TIAN Q, LUO J P, LIU X H, et al.. Development and application of rapid detecting instrument for bacteria based on bioluminescence [J]. Opt. Precision Eng., 2010, 18(4): 771-778. (in Chinese)

[12] 吴太虎, 毛佳文, 陈锋, 等. 痕量微生物快速检测系统[J].光学 精密工程, 2015, 23(11): 3061-3068.

    Wu T H, MAO J W, CHEN F, et al.. Rapid trace microbia detection system [J].Opt. Precision Eng., 2015, 23(11): 3061-3068. (in Chinese)

[13] LI X T, JIANG K, WANG P. Research of quantitative analyzer for fluorescence immune chromatography strips[J].Applied Mechanics & Materials, 2013, 373-375: 1000-1006.

[14] 顾雯雯. 微流控细胞芯片LED诱导透射式荧光检测微系统[J].光学 精密工程, 2014, 22(8): 2159-2165.

    Gu W W. LED induced transmitted fluorescence detector integrated in microfluidic cell chip [J]. Opt. Precision Eng., 2014, 22(8): 2159-2165. (in Chinese)

[15] 李庆扬, 王能超, 易大义. 数值分析[M]. 第五版.北京: 清华大学出版社, 2014.

    LI Q Y, WANG N CH, YI D Y. Numeric Analysis [M]. Fifth Edition. Beijing: Tsinghua University Press, 2014. (in Chinese)

[16] 肖友益. 化学发光免疫分析标准曲线拟合模式选择[J].现代检验医学杂志, 2009, 24(5): 157.

    XIAO Y Y. Selection of chemiluminescence immune assay standard curve fitting model [J]. Journal of Modern Laboratory Medicine, 2009, 24(5): 157. (in Chinese)

董国亚, 赵翔, 张燕, 孙传强. 便携式上转换荧光试纸条检测仪的研制[J]. 光学 精密工程, 2017, 25(3): 584. DONG Guo-ya, ZHAO Xiang, ZHANG Yan, SUN Chuan-qiang. Development of portable up-conversion photoluminescence strip detector[J]. Optics and Precision Engineering, 2017, 25(3): 584.

本文已被 5 篇论文引用
被引统计数据来源于中国光学期刊网
引用该论文: TXT   |   EndNote

相关论文

加载中...

关于本站 Cookie 的使用提示

中国光学期刊网使用基于 cookie 的技术来更好地为您提供各项服务,点击此处了解我们的隐私策略。 如您需继续使用本网站,请您授权我们使用本地 cookie 来保存部分信息。
全站搜索
您最值得信赖的光电行业旗舰网络服务平台!