Chinese Optics Letters, 2018, 16 (11): 111801, Published Online: Nov. 11, 2018
Sidelobe suppression in light-sheet fluorescence microscopy with Bessel beam plane illumination using subtractive imaging Download: 737次
Figures & Tables
Fig. 2. Calculated intensity distributions of (a), (c) the extended Bessel light-sheet beam and (b), (d) the ring-like Bessel light-sheet beam (a), (b) in the y – z plane with x = 0 and (c), (d) in the x – z plane with y = 0 . (e) The normalized line profiles of the intensity distributions along the central z axis in (c) and (d).
Fig. 3. PSFs of the light-sheet microscopy in the y – z plane. (a) The calculated illumination PSF for the conventional Bessel LSFM with an annular mask characterized by NA max = 0.7 and NA min = 0.68 . (b) The detection PSF with NA det = 0.7 . (c) The system PSF for the conventional Bessel LSFM. (d) The system PSF for subtractive Bessel LSFM obtained with extended solid Bessel beam with an annular mask characterized by NA max = 0.67 and NA min = 0.65 and a ring-like Bessel beam with an annular mask characterized by NA max = 0.7 and NA min = 0.68 . (e) The normalized line profiles of the intensity distributions along the central axis in (c) and (d).
Fig. 4. Simulated images of a sample consisting of 16 points shown in (a) for (b) the conventional Bessel LSFM and (c) the subtractive Bessel LSFM.
Suhui Deng, Yiping Xiao, Jie Hu, Jianfang Chen, Yuhao Wang, Mingping Liu. Sidelobe suppression in light-sheet fluorescence microscopy with Bessel beam plane illumination using subtractive imaging[J]. Chinese Optics Letters, 2018, 16(11): 111801.