激光与光电子学进展, 2014, 51 (12): 122402, 网络出版: 2014-11-07  

利用一种光反射差法同时监测两种蛋白的结合反应

Real-Time Binding Reactions Detected by Optical Reflectivity Difference from Blended Protein Samples
王旭 1,2,*孔艳 1朱焯炜 1
作者单位
1 江南大学理学院, 江苏 无锡 214122
2 加州大学戴维斯分校物理系, 美国加利福尼亚州 95616
摘要
在检测未知样品中是否含有某种病毒或细菌时,通常可以利用该病毒或细菌的已知特异性探针来检测未知病毒或细菌的抗原成分。但通常单一样品中也会有多种病毒或细菌,利用各种已知探针逐个检测样品效率较低,有必要发展新的检测方式提高检测通量。这里进行了利用两种探针混合液同时检测同一样品中两种蛋白的实验。实验中利用一种光反射差方法实时监测样品点与混合探针的反应曲线,通过对曲线的分析拟合,可以确认样品中有两类蛋白存在。实验结果证实,利用该反射差法结合混合探针能更高效地检测含有病毒或细菌的样品,可能是提高检测通量的一种方式。
Abstract
Most methods used in virus and bacterium detection are based on confirmation of reactions between unknown samples and known probes. Since several bacterium species can survive in one sample, these methods have to be used to perform detections one by one with different probes, Which are with low efficiency. An experiment using mixed probes to detect two blended proteins at the same time is designed. By monitoring the binding curves of these two probe-target reactions with an optical reflectivity difference method, it is explicit whether there are two different proteins in the sample. The demonstration experiments show potentials of this method for higher throughput detection of viruses or bacteria.
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王旭, 孔艳, 朱焯炜. 利用一种光反射差法同时监测两种蛋白的结合反应[J]. 激光与光电子学进展, 2014, 51(12): 122402. Wang Xu, Kong Yan, Zhu Zhuowei. Real-Time Binding Reactions Detected by Optical Reflectivity Difference from Blended Protein Samples[J]. Laser & Optoelectronics Progress, 2014, 51(12): 122402.

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