以银纳米棒/氯化银为SERS基底用维多利亚蓝B分子探针检测大肠杆菌

梁爱惠; 王耀辉; 欧阳辉祥; 温桂清; 张杏辉; 蒋治良

doi:doi:10.3964/j.issn.1000-0593(2017)11-3446-03

光谱学与光谱分析, 2017, 37 (11): 3446, 网络出版: 2018-01-04

以银纳米棒/氯化银为SERS基底用维多利亚蓝B分子探针检测大肠杆菌

Vitoria Blue B SERS Molecular Probe Detection of Escherichia coli in the Silver Nanorod/AgCl Sol Substrate

论文大纲

梁爱惠 ^*王耀辉欧阳辉祥温桂清张杏辉蒋治良

作者单位

珍稀濒危动植物生态与环境保护教育部重点实验室, 广西师范大学, 广西桂林 541004

大肠杆菌维多利亚蓝B染色银纳米棒增敏剂 Escherichia coli Vitoria blue B Dying Silver nanorod Enhancer SERS SERS

摘要

加入增敏剂AgNO3和NaCl, 在银纳米棒（AgNRs）表面吸附了较牢固的AgCl并形成高SERS活性的AgNR/AgCl溶胶基底, 维多利亚蓝B（VBB）分子探针在1 611 cm-1处有一较强的SERS峰。用VBB做大肠杆菌(EC)的染色剂, 使染色的大肠杆菌具备VBB分子探针的SERS特性, 即VBB染色大肠杆菌也在1 611 cm-1处有一较强的SERS峰。在最优条件下, 该SERS峰强与大肠杆菌浓度在5×106～3×109 cfu·mL-1 范围内成正比, 检出限为2×106 cfu·mL-1, 用于水样和饮料中大肠杆菌的分析, 具有简便、快速、灵敏等优点。

Abstract

Using AgNO3 and NaCl as enhnacers, highly SERS active AgNRs/AgCl nanosol substrate were prepared, in which the hydrophobic AgCl molecules formed and absorbed on the AgNRs surface by mens of strong hydrophobic forces between AgNRs and AgCl. The Vitoria blue B molecular probes on the surfaces of AgNRs/AgCl displayed a strong SERS paek at 1 611 cm-1. VBB was used to dye the Escherichia coli (E. coli), which exhibited the SERS proprerty with a Raman peak at 1 611 cm-1. Under the optimal conditions, the SERS intensities were linear to E. coli concentration in the range of 5×106～3×109 cfu·mL-1, with a detection of 2×106 cfu·mL-1. The new SERS method was applied for detection of E. coli in real samples, with features such as simplicity, rapidity and sensitivity. The results were in agreement with that of plate-count method.

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梁爱惠, 王耀辉, 欧阳辉祥, 温桂清, 张杏辉, 蒋治良. 以银纳米棒/氯化银为SERS基底用维多利亚蓝B分子探针检测大肠杆菌[J]. 光谱学与光谱分析, 2017, 37(11): 3446. LIANG Ai-hui, WANG Yao-hui, OUYANG Hui-xiang, WEN Gui-qing, ZHANG Xing-hui, JIANG Zhi-liang. Vitoria Blue B SERS Molecular Probe Detection of Escherichia coli in the Silver Nanorod/AgCl Sol Substrate[J]. Spectroscopy and Spectral Analysis, 2017, 37(11): 3446.

以银纳米棒/氯化银为SERS基底用维多利亚蓝B分子探针检测大肠杆菌

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