激光生物学报, 2018, 27 (5): 442, 网络出版: 2018-11-25  

脂肪酶lipAB操纵子在防御假单胞菌中的克隆、表达及活性研究

Cloning, Expression and Activity of Lipase Operon lipAB in Pseudomonas protegens
作者单位
1 淡水鱼类发育生物学国家重点实验室, 湖南师范大学生命科学学院, 微生物分子生物学湖南省重点实验室, 湖南 长沙 410081
2 山东省土壤肥料总站, 山东 济南250100
3 山东大学生命科学学院, 山东大学亥姆霍兹生物技术研究所, 微生物技术国家重点实验室, 山东 青岛 266237
4 山东大学苏州研究院, 江苏 苏州 215123
引用该论文

方倩, 谢芝玲, 陈汉娜, 李建伟, 潘登, 丁学知, 夏立秋, 涂强, 张友明. 脂肪酶lipAB操纵子在防御假单胞菌中的克隆、表达及活性研究[J]. 激光生物学报, 2018, 27(5): 442.

FANG Qian, XIE Zhiling, CHEN Hanna, LI Jianwei, PAN Deng, DING Xuezhi, XIA Liqiu, TU Qiang, ZHANG Youming. Cloning, Expression and Activity of Lipase Operon lipAB in Pseudomonas protegens[J]. Acta Laser Biology Sinica, 2018, 27(5): 442.

参考文献

[1] PANDEY A, BENJAMIN S, SOCCOL C R, et al. The realm of microbial lipases in biotechnology[J]. Biotechnology and Applied Biochemistry, 1999, 29(Pt 2):119-131.

[2] VERMA M L, AZMI W, KANWAR S S. Microbial lipases:at the interface of aqueous and non-aqueous media. a review[J]. Acta Microbiol Immunol Hung, 2008, 55(3):265-294.

[3] GHOSH P K, SAXENA R K, GUPTA R, et al. Microbial lipases:production and applications[J]. Science Program, 1996, 79(Pt 2):119-157.

[4] JUNG W S, LEE J, KIM M I, et al. Structural and functional analysis of phytotoxin toxoflavin-degrading enzyme[J]. PloS One, 2011, 6(7):e224-243.

[5] JEONG Y, KIM J, KIM S, et al. Toxoflavin produced by Burkholderia glumae causing rice grain rot is responsible for inducing bacterial wilt in many field crops[J]. Plant Diseases, 2003, 87(8):890-895.

[6] OLLIS D L, CHEAH E, CYGLER M, et al. The alpha/beta hydrolase fold[J]. Protein Engineerin, 1992, 5(3):197-211.

[7] LANG D, HOFMANN B, HAALCK L, et al. Crystal structure of a bacterial lipase from Chromobacterium viscosum ATCC 6918 refined at 1.6 angstroms resolution[J]. Journal of Molecular Biology, 1996, 259(4):704-717.

[8] JAEGER K E, RANSAC S, DIJKSTRA B W, et al. Bacterial lipases:reviews[J]. FEMS Microbiology Letters, 1994, 15(1):29-63.

[9] FRENKEN L G, EGMOND M R, BATENBURG A M, et al. Cloning of the Pseudomonas glumae lipase gene and determination of the active site residues[J]. Applied and Environmental Microbiology, 1992, 58(12):3787-3791.

[10] BANEYX F, MUJACIC M. Recombinant protein folding and misfolding in Escherichia coli:review[J]. Nature Biotechnology, 2004, 22:1399-1408.

[11] RAMETTE A, FRAPOLLI M, FISCHERLE M S, et al. Pseudomonas protegens sp. nov., widespread plant-protecting bacteria producing the biocontrol compounds 2, 4-diacetylphloroglucinol and pyoluteorin[J]. Systematic and Applied Microbiology, 2011, 34(3):180-188.

[12] NAGARAJKUMAR M, JAYARAJ J, MUTHUKRISHNAN S, et al. Detoxific-ation of oxalic acid by Pseudomonas fluorescens strain PfMDU2:implications for the biological control of rice sheath blight caused by Rhizoctonia solani[J]. Microbiological Research, 2005, 160(3):291-298.

[13] 孙广正, 姚拓, 赵桂琴, 等. 荧光假单胞菌防治植物病害研究现状与展望[J]. 草业学报, 2015, 24(4):174-190.

    SUN Guangzheng, YAO Tuo, ZHAO Guiqin, et al. Research progress and prospects for controlling plant disease susing Pseudomonas flurescens[J]. Acta Prataculturae Sinica, 2015, 24(4):174-190.

[14] ZHA D, XU L, ZHANG H, et al. Molecular identification of lipase LipA from Pseudomonas protegens Pf-5 and characterization of two whole-cell biocatalysts Pf-5 and Top10lipA[J]. Journal of Microbiology and Biotechnology, 2014, 24(5):619-628.

[15] ZHA D, ZHANG H, ZHANG H, et al. N-terminal transmembrane domain of lipase LipA from Pseudomonas protegens Pf-5:a must for its efficient folding into an active conformation[J]. Biochimie, 2014, 105:165-171.

[16] WANG H, LI Z, JIA R, et al. RecET direct cloning and Redαβ recombineering of biosynthetic gene clusters, large operons or single genes for heterologous expression[J]. Nature Protocols, 2016, 11(7):1175-1190.

[17] ZHANG Y, MUYRERS J P, TESTA G, et al. DNA cloning by homologous recombination in Escherichia coli[J]. Nature Biotechnology, 2000, 18(12):1314-1317.

[18] ZHANG Y, BUCHHOLZ F, MUYRERS J P, et al. A new logic for DNA engineering using recombination in Escherichia coli[J]. Nature Genetics, 1998, 20(2):123-128.

[19] WANG J, SAROV M, RIENTJES J, et al. An improved recombineering approach by adding RecA to lambda red recombination[J]. Journal of Microbiology and Biotechnology, 2006, 32(1):43-53.

[20] FU J, BIAN X, HU S, et al. Full-length RecE enhances linear-linear homologous recombination and facilitates direct cloning for bioprospecting[J]. Nature Biotechnology, 2012, 30(5):440-446.

[21] 郑文韬, 张友明, 卞小莹. Red/ET同源重组技术及其在微生物基因组挖掘中的应用进展[J]. 微生物学报, 2017, 57(11):1735-1746.

    ZHENG Wentao, ZHANG Youming, BIAN Xiaoying. Advances in Red/ET recombineering and its application for microbial genome mining[J]. Acta Microbiologica Sinica, 2017, 57(11):1735-1746.

[22] TU Q, YIN J, FU J, et al. Room temperature electrocompetent bacterial cells improve DNA transformation and recombineering efficiency[J]. Scientific Reports, 2016, 20(6):246-248.

[23] 邬敏辰, 孙崇荣, 邬显章. 平板扩散法粗略确定碱性脂肪酶的活性[J]. 无锡轻工大学学报, 2000, 19(2):168-172.

    WU Minchen, SUN Congrong, WU Xianzhang. Diffusion plate assay for quick and rough estimation of alkaline lipase activity[J]. Journal of Wuxi University of Light Industry, 2000, 19(2):168-172.

[24] GALLE P, JACQUES C B. Hydrolysis of p-nitrophenyl palmitate in n-heptane by the Pseudomonas cepacia lipase:a simple test for the determination of lipase activity in organic media[J]. Enzyme and Microbial Technology, 1996, 18:417-422.

[25] 滕昆, 张吉福, 牛福星, 等. 脂肪酶活力测定中终止反应方法的比较分析[J]. 广西科学, 2014, 21(2):115-118.

    TENG Kun, ZHANG Jifu, NIU Fuxing, et al. Comparative analysis of termination reaction methods in lipase activity assay[J]. Guangxi Science, 2014, 21(2):115-118.

方倩, 谢芝玲, 陈汉娜, 李建伟, 潘登, 丁学知, 夏立秋, 涂强, 张友明. 脂肪酶lipAB操纵子在防御假单胞菌中的克隆、表达及活性研究[J]. 激光生物学报, 2018, 27(5): 442. FANG Qian, XIE Zhiling, CHEN Hanna, LI Jianwei, PAN Deng, DING Xuezhi, XIA Liqiu, TU Qiang, ZHANG Youming. Cloning, Expression and Activity of Lipase Operon lipAB in Pseudomonas protegens[J]. Acta Laser Biology Sinica, 2018, 27(5): 442.

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