1 上海大学 机电工程与自动化学院, 上海 200444
2 中国科学院 苏州生物医学工程技术研究所光健康研究中心, 江苏 苏州 215163
3 解放军总医院 第三医学中心, 北京 100039
为了将荧光分子成像技术应用于临床进行手术导航、肿瘤边界识别、在体显微病理诊断等, 设计了一种双模切换显微内窥镜成像系统, 采用荧光素钠作为荧光分子探针, 高亮度蓝光LED光源作为荧光激发光源, 通过切换内窥成像探头, 实现了两个模态下的成像: 宽场白光内窥成像模式下进行手术导航, 荧光分子成像进行病变肿瘤边界识别; 显微内窥成像模式下, 进行在体显微病理分析, 确定肿瘤良恶性及其种类。本文研究了双模切换显微内窥镜成像系统的光学特性, 搭建系统并测试了相关的性能指标, 展示了该系统在小鼠肝脏多模式内窥成像下的效果。研究结果表明: 宽场内窥成像可以实现组织颜色和边界特征识别, 显微内镜成像系统可以实现分辨率达4.4 μm的组织显微成像, 能够满足在体肿瘤实时手术导航和显微病理诊断的临床需求。
肿瘤 内镜检查 边界特征识别 荧光成像 tumor endoscopy LED boundary feature recognition LED fluorescence imaging
Author Affiliations
Abstract
1 Department of Control Science and Engineering, Harbin Institute of Technology, Harbin 150001, China
2 Department of Gastroenterology, General Hospital of Chinese People’s Armed Police Forces, Beijing 100039, China
3 Institute of Opto-electronics, Harbin Institute of Technology, Harbin 150080, China
Photoacoustic tomography (PAT) has the unique capability of visualizing optical absorption inside several centimeters-deep biological tissue with a high spatial resolution. However, single linear-array transducer-based PAT suffers from the limited-view challenge, and thus the synthetic aperture configuration is designed that still requires multichannel data acquisition hardware. Herein, a feasible synthetic aperture PAT based on compressed sensing reconstruction is proposed. Both the simulation and experimental results tested the theoretical model and validated that this approach can improve the image resolution and address the limited-view problem while preserving the target information with a fewer number of measurements.
110.5120 Photoacoutic imaging 100.3020 Image reconstruction-restoration 170.5120 Photoacoustic imaging Chinese Optics Letters
2017, 15(10): 101102
