上转换荧光材料由于具有独特的反斯托克斯发射、激发光成像深、自发荧光低、物理化学性质稳定等特点，因此作为影像药剂被广泛研究，在生物医疗领域的应用也得到了不断的发展，但较低的发光效率制约了其发展。金属纳米粒子具有独特的局域表面等离子共振效应，可以有效地增强上转换荧光。本文主要综述离散偶极近似仿真对金属调制荧光的理论指导、实验验证和生物应用。离散偶极近似仿真能够计算纳米粒子电磁特性，可以预测等离子共振峰的位置和强度，从而指导设计金属增强稀土荧光的结构，并对其荧光性能进行测试验证。综述中共涉及了六种不同的稀土-金属复合结构，都获得了金属增强荧光的效果，并实现了其在生物成像和治疗领域的应用。

为了实现小动物光声断层信号的高速采集和实时高质量图像的重建, 采用了覆盖角度为270°的128阵元弧形聚焦超声换能器、4个32通道的NI公司数据采集模块和可调谐脉冲激光器以及正则化优化的基于模型的光声断层重建算法。结果表明, 系统的空间分辨率可以达到180μm;此系统可以在1ms内完成光声断层数据的采集, 在40s以内获得高质量的重建图像。该系统可以用于开展小动物在体的多光谱光声断层成像实验研究。

We present a sparse Bayesian reconstruction method based on multiple types of a priori information for multispectral bioluminescence tomography (BLT). In the Bayesian approach, five kinds of a priori information are incorporated, reducing the ill-posedness of BLT. Specifically, source sparsity characteristic is considered to promote reconstruction results. Considering the computational burden in the multispectral case, a series of strategies is adopted to improve computational efficiency, such as optimal permissible source region strategy and node model of the finite element method. The performance of the proposed algorithm is validated by a heterogeneous three-dimensional (3D) micron scale computed tomography atlas and a mouse-shaped phantom. Reconstructed results demonstrate the feasibility and effectiveness of the proposed algorithm.