研究了经三种不同类型的透明剂处理后的在体人皮组织在波长为400～1 000 nm范围内随时间变化反射光谱特性的变化及各组于580 nm处分别在0～60 min时间段各组反射光谱的改变程度。 实验采用USB-4000光纤光谱仪分别获取经丙三醇、 葡萄糖及丙二醇处理在体人皮肤组织前后的在0, 10, 20, 30, 40, 50和60 min的平均漫反射光谱。 实验结果表明: 分别经三种不同类型透明处理后的皮肤组织的平均反射光强度都呈现出明显的减弱， 但是随着各自作用于皮肤组织时间的增长， 所导致的相对反射率减少量逐渐增大。 经40%甘油作用于皮肤组织后在10, 20, 30, 40， 50和60 min处的相对反射率的减少分别为5%, 7%, 9%, 10%, 11%和12%， 经40%葡萄糖作用于皮肤组织后在10, 20, 30, 40, 50和 60 min处的相对反射率的减少分别为9%, 13%, 16%, 19%, 21%和22%， 而40%丙二醇作用于皮肤组织后在10, 20, 30, 40, 50和60 min处的相对反射率的减少分别为14%, 22%, 29%, 32%, 34%和35%。 可见， 这三种不同类型的透明剂都能够显著增强光在皮肤组织中的穿透能力， 在0～60 min的时间内各组得光透明时间进程也有所不同， 在同体积分数下， 光学透明效果差异显著地由高到低依次为40%丙二醇， 40%葡萄糖， 40%甘油。

In order to enhance the optical clearing effect of topically applied optical clearing agents (OCAs), we evaluated the effect of propylene glycol (PG) as a chemical penetration enhancer (PE) on optical clearing of skin in vitro by observation and measurement of optical-transmittance and diffuse-reflectance spectra. Three OCAs, i.e., glycerol, D-sorbitol and PEG400, and two other penetration enhancers, Azone and Thiazone, were used in this study. The results indicated that the decrease of reduced scattering coefficient caused by OCA/PG was larger than that by pure OCA, and the change by OCA/water was the least after the same treatment time. There were significant differences for the reduced scattering coefficient at 630 nm after 120 min application of agents between OCA and OCA/PG. The efficacy of optical clearing caused by OCA/PG depended on the OCA itself. When PEG400 was mixed with three different PEs, we found the optical clearing were different. The penetration enhancing ability of PG was much better compared to Azone, and suboptimal to Thiazone. Also, this study provides evidence for the use of PG as a PE in order to improve skin optical clearing.