采用高精度纳米位移台作为被测振动体目标,研究基于超小自聚焦(GRIN)光纤探头的扫频光学相干层析成像(SS-OCT)测振技术。解析基于超小GRIN光纤探头的集成化SS-OCT工作性能,理论上该系统可测振动的最大峰峰值达12.5 mm,最大频率达25 kHz。搭建相应的测振实验系统,并进行微小振动的测试及分析。结果显示,设置纳米位移台振动峰峰值范围1 nm~5 μm、频率范围1~200 Hz,该集成化SS-OCT测振系统可测出频率1 Hz、峰峰值6 nm及以上的微小振动。对振幅为25 nm、频率为10Hz的单频正弦振动信号,系统的重复性实验标准偏差为0.003。结果表明,基于超小GRIN光纤探头的集成化SS-OCT系统具有测量微小振动的可行性,为进一步研究其在微小振动和位移等精确测量领域的应用提供了实验基础。

Cerebral edema is a severe complication of acute ischemic stroke with high mortality but limited treatment. Although parameters such as brain water content and intracranial pressure may represent the global assessment of edema, optical properties can appear heterogeneously throughout the cerebral tissue relative to the site of injury. In this study, we have monitored the edema formation and progression in both permanent and transient middle cerebral artery occlusion models in rats. Edema was reflected by the decrease of optical attenuation coefficient (OAC) value in OCT system. By utilizing swept-source optical coherence tomography (SS-OCT), we found that in photochemically induced permanent focal stroke model, both the edema size and edema index, steadily developed until the end of monitor (7 h). Comparatively, when transient ischemia was introduced with endothelin-1 (ET-1), the edema was detected as early as 15 min, and began to recover after 30 min until monitor was finished (3 h). Despite the majority of the edema being recovered to some extent, the condition of a small region within the edema kept deteriorating, presumably due to the reperfusion damage which might result in serious clinical outcomes. Our study has compared the edema characteristics from two different acute ischemic stroke situations. This work not only confirms the capability of OCT to temporal and spatial monitor of edema but is also able to locate focal conditions at some areas that might highly determine the prognosis and treatment decisions.

Accurate segmentation of choroidal thickness (CT) and vasculature is important to better analyze and understand the choroid-related ocular diseases. In this paper, we proposed and implemented a novel and practical method based on the deep learning algorithms, residual U-Net, to segment and quantify the CT and vasculature automatically. With limited training data and validation data, the residual U-Net was capable of identifying the choroidal boundaries as precise as the manual segmentation compared with an experienced operator. Then, the trained deep learning algorithms was applied to 217 images and six choroidal relevant parameters were extracted, we found high intraclass correlation coefficients (ICC) of more than 0.964 between manual and automatic segmentation methods. The automatic method also achieved great reproducibility with ICC greater than 0.913, indicating good consistency of the automatic segmentation method. Our results suggested the deep learning algorithms can accurately and efficiently segment choroid boundaries, which will be helpful to quantify the CT and vasculature.

利用随机相矢量和模型,描述了光学相干层析术(OCT)结构信号的强度概率密度分布,推导出振幅差分信号的概率密度分布,从理论上确定了差分图像的动态与静态区域的阈值模型。利用一套可对人体皮肤的微血管进行扫描成像的扫频光学相干层析系统,通过阈值模型生成二值化图像,将实验室已有的基于体数据的互相关图像与二值化图像相结合。结果表明该模型有助于减小图像的散斑噪声,从而获得更清晰的结构信息。

当前墨迹伪造造成的经济损失逐年增加,基于此,提出一种基于扫频光学相干层析(SSOCT)的墨迹鉴别方法。使用自主搭建的SSOCT成像系统采集墨迹样品背向反射光强随深度下降的数据,通过数据拟合获得墨迹层的衰减系数,用以区分同种颜色不同品牌墨水在白纸上书写的墨迹。实验测得百乐牌墨迹衰减系数范围为0.1333~0.1434,得力牌墨迹衰减系数范围为0.1501~0.1695,英雄牌墨迹衰减系数范围为0.1748~0.1892。重建了使用三种墨水书写的“OCT”字母的X-Y横截面图像,实现了墨迹的鉴别与区分,验证了方法的可行性。

提出一种基于扫频光学相干层析术(SSOCT)的重建人体皮下微血管三维结构的新算法。该方法利用搭建的手持式人体皮肤微血管扫频光学相干层析系统,采用多帧B扫描对数强度血流图像微分标准偏差计算,获取人体皮肤在体截面图像和微血管正面图(en face image)。实验结果证明,与现有的对数强度微分标准偏差算法(DSDLI)相比,本文算法能够更好地恢复出血流图像信息,在重建图像中可以明显看到原算法的处理结果中看不出的血流细节,更有利于光学相干层析术(OCT)系统在癌症、糖尿病、酒色斑等疾病在医学诊断方面的现实应用。

报道了一种可实现入瞳位置不变的超广角扫频光相干层析成像(OCT)系统,对成像系统的样品臂进行了建模和光学仿真, 证实其可行性和有效性。设计并展示了一个超广角OCT成像系统, 在仅改变振镜位置及透镜间距的情况下, 实现了超过40°的眼底单次超大角度扫描, 单次横向扫描范围覆盖眼底I区, 有效增加了OCT单次扫描的成像范围。

Melanoma, characterized by high mortality, rapid development and accompanied with angiogenesis is the most typical malignant tumor in skin cancer. Hence, the detection of blood vessels is of much significance. The early vascular network has small scale. If we remove the tumor early and biopsy it, it will increase the spread of the cancer cells and infection and bleeding. In this case, we presented a new angiography method. A high-resolution OCT system for noninvasive angiographic imaging of early skin melanoma — Swept Source Optical Coherence Tomography Angiography (SS-OCTA) is proposed. With a high lateral resolution of 10 μm in vivo tomographic angiography, SS-OCTA is used to image and identify the morphology of the early tumor blood vessels. In addition, a control group experiment is conducted to observe the growth of melanoma in the process of rupture, malformation of micro-vessels. The results of the analysis and statistical test (P < 0:05) are statistically significant.