四种藏药成方制剂中铅、砷含量的湿法消化-流动注射氢化物发生-原子吸收光谱法测定研究

郑植元; 杜玉枝; 张明; 于明杰; 李岑; 杨红霞; 赵静; 夏政华; 魏立新

doi:doi:10.3964/j.issn.1000-0593(2015)04-1037-06

光谱学与光谱分析, 2015, 35 (4): 1037, 网络出版: 2015-04-20

四种藏药成方制剂中铅、砷含量的湿法消化-流动注射氢化物发生-原子吸收光谱法测定研究

Study on Content Determination of Lead and Arsenic in Four Traditional Tibetan Medicine Prescription Preparations by Wet Digestion Flow Injection-Hydride Generation-Atomic Absorption Spectrometry

论文大纲

郑植元 ^1,2,3,*杜玉枝 ^1,2张明 ^1,2,3于明杰 ^1,2,3李岑 ^1,2,3杨红霞 ^1,2赵静 ^1,2,3夏政华 ^1,2,3魏立新 ^1,2

作者单位

¹ 中国科学院西北高原生物研究所, 青海西宁 810001

² 青海省藏药药理学和安全性评价研究重点实验室, 青海西宁 810001

³ 中国科学院大学, 北京 100049

摘要

选择藏族地区常用的四种藏药成方制剂安置精华散、当佐、仁青常觉和七十味珍珠丸作为研究对象,通过优化消化条件和测定条件,探索建立其铅、砷含量测定的湿法消化与流动注射氢化物发生-原子吸收光谱(FI-HAAS)联用分析方法,并对其铅砷含量进行精确测定.在优化的工作条件下,铅和砷的检出限分别为:0.067和0.012 μg·mL-1;定量限分别为:0.22和0.041 μg·mL-1;线性范围分别为:25～1 600 ng·mL-1(r=0.999 5),12.5～800 ng·mL-1(r=0.999 4);精密度(RSD)分别为:2.0%和3.2%;加标回收率范围分别为:98.00%～99.98%和96.67%～99.87%.四种藏药成方制剂的铅、砷含量测定结果如下,安置精华散中铅砷含量分别为:0.63～0.67和0.32～0.33 μg·g-1;当佐为:42.92～43.36和24.67～25.87 μg·g-1;仁青常觉为:1 611.39～1 631.36和926.76～956.52 μg·g-1;七十味珍珠丸为:1 102.28～1 119.27和509.96～516.87 μg·g-1.本研究建立了藏药成方制剂中铅、砷检测方法,并测定了四种藏药成方制剂中铅、砷含量,为其在临床安全有效使用提供了参考依据.

Abstract

Four common traditional tibetan medicine prescription preparations “Anzhijinghuasan,Dangzuo,Renqingchangjue and Rannasangpei” in tibetan areas were selected as study objects in the present study.The purpose was to try to establish a kind of wet digestion and flow injection-hydride generation-atomic absorption spectrometry (FI-HAAS) associated analysis method for the content determinations of lead and arsenic in traditional tibetan medicine under optimized digestion and measurement conditions and determine their contents accurately.Under these optimum operating conditions,experimental results were as follows.The detection limits for lead and arsenic were 0.067 and 0.012 μg·mL-1 respectively.The quantification limits for lead and arsenic were 0.22 and 0.041 μg·mL-1 respectively.The linear ranges for lead and arsenic were 25～1 600 ng·mL-1 (r=0.999 5) and 12.5～800 ng·mL-1(r=0.999 4) respectively.The degrees of precision(RSD) for lead and arsenic were 2.0% and 3.2% respectively.The recovery rates for lead and arsenic were 98.00%～99.98% and 96.67%～99.87% respectively.The content determination results of lead and arsenic in four traditional tibetan medicine prescription preparations were as follows.The contents of lead and arsenic in Anzhijinghuasan are 0.63～0.67 μg·g-1 and 0.32～0.33 μg·g-1in Anzhijinghuasan,42.92～43.36 μg·g-1 and 24.67～25.87 μg·g-1 in Dangzuo,1 611.39～1 631.36 μg·g-1 and 926.76～956.52 μg·g-1 in Renqing Changjue,and 1 102.28～1 119.27 μg·g-1 and 509.96～516.87 μg·g-1 in Rannasangpei,respectively.This study established a method for content determination of lead and arsenic in traditional tibetan medicine,and determined the content levels of lead and arsenic in four tibetan medicine prescription preparations accurately.In addition,these results also provide the basis for the safe and effective use of those medicines in clinic.

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郑植元, 杜玉枝, 张明, 于明杰, 李岑, 杨红霞, 赵静, 夏政华, 魏立新. 四种藏药成方制剂中铅、砷含量的湿法消化-流动注射氢化物发生-原子吸收光谱法测定研究[J]. 光谱学与光谱分析, 2015, 35(4): 1037. ZHENG Zhi-yuan, DU Yu-zhi, ZHANG Ming, YU Ming-jie, LI Cen, YANG Hong-xia, ZHAO Jing, XIA Zheng-hua, WEI Li-xin. Study on Content Determination of Lead and Arsenic in Four Traditional Tibetan Medicine Prescription Preparations by Wet Digestion Flow Injection-Hydride Generation-Atomic Absorption Spectrometry[J]. Spectroscopy and Spectral Analysis, 2015, 35(4): 1037.

四种藏药成方制剂中铅、砷含量的湿法消化-流动注射氢化物发生-原子吸收光谱法测定研究

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