激光与光电子学进展, 2020, 57 (12): 120001, 网络出版: 2020-06-03
自适应光学在荧光显微镜中的应用 
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Application of Adaptive Optics in Fluorescence Microscope
图 & 表
图 2. AO宽场显微镜系统及AO校正前后成像对比[23]。(a)基于SHWS、荧光微球和DM的AO宽场显微镜系统;(b) AO校正前后绿色荧光珠的宽场成像
Fig. 2. AO wide-field microscope system and comparison of wide-field images before and after AO correction[23]. (a) Schematic of AO wide-field microscope system based on SHWS, fluorescent beads, and DM; (b) wide-field images of green fluorescent beads before and after AO correction
图 3. 共轭AO宽场显微镜系统及AO校正前后成像对比。(a)基于PAW的共轭AO宽场显微镜系统[26]; (b)共轭AO校正前后荧光标记的异常小鼠肾脏切片荧光图像[27]
Fig. 3. Conjugate AO wide-field microscope system and comparison of images before and after AO correction. (a) Schematic of PAW-based conjugate AO wide-field microscope system[26]; (b) fluorescence images of aberrated fluorescently-labeled mouse kidney section without and with conjugate AO correction[27]
图 4. AOSIM系统及AO校正前后成像对比[33]。(a) Woofer-tweeter AOSIM系统;(b) AO校正前后果蝇胚胎中GFP标记的aCC/RP2运动神经元的宽场和SIM图像
Fig. 4. AOSIM system and comparison of images before and after AO correction[33]. (a) Schematic of woofer-tweeter AOSIM system; (b) wide field and SIM images of GFP-labeled aCC/RP2 motoneurons of a Drosophila embryo before and after AO correction
图 5. 两种AO单分子定位显微镜系统。(a)基于WFS和DM的AO光激活定位显微镜系统[35];(b)基于DM和图像锐度度量的无波前传感器反馈AO单分子开关显微镜系统[37]
Fig. 5. Schematics of two AO single molecule location microscope systems. (a) Schematic of AO-PALM system based on WFS and DM[35]; (b) schematic of wavefront sensorless feedback AO single molecule switch microscope based on DM and image sharpness measurement[37]
图 6. 基于搜索算法的AO-STORM系统对细胞微管的像差校正。(a)利用基于GA的AO-STORM系统对hepG2细胞微管进行像差校正[39];(b)利用基于PSO算法的AO-STORM系统对Hela细胞微管进行像差校正[40]
Fig. 6. Aberration correction of microtubules by AO-STORM system based on search algorithm. (a) Aberration correction using GA-based AO-STORM system on microtubules of hepG2 cell[39]; (b) aberration correction using PSO-based AO-STORM system on microtubules of Hela cell[40]
图 7. AO共聚焦显微镜系统及AO校正前后成像对比[42]。(a)基于SHWS、荧光微球和DM的AO共聚焦荧光显微镜系统;(b) 100 μm厚小鼠脑组织AO校正前后的共聚焦荧光成像
Fig. 7. AO confocal microscope system and comparison of images before and after AO correction[42]. (a) Schematic of AO confocal fluorescence microscope based on SHWS, fluorescent beads, and DM; (b) confocal fluorescence images through 100 μm thick mouse brain tissue before and after AO correction
图 8. 基于DM和SPGD算法的无波前传感器AO共聚焦荧光显微镜系统[46]
Fig. 8. Schematic of wavefront sensorless AO confocal fluorescence microscope based on DM and SPGD algorithm[46]
图 9. 基于导引星全息的DAO共聚焦显微镜系统[52]
Fig. 9. Schematic of DAO confocal microscope based on guide star hologram[52]
图 10. 共聚焦显微镜畸变测量[53]。 (a) SLM上的孔径分割示意图;(b)通过聚焦两个圆形子区域的光产生的干涉图案
Fig. 10. Aberration measurement by confocal microscope[53]. (a) Pupil segmentation on SLM; (b) interference patterns generated by focusing light from two selected circular segments
图 11. AO双光子显微镜系统及AO校正前后成像对比[57]。(a)基于SHWS和开环控制的AO双光子显微镜系统;(b)AO校正前后活体果蝇胚胎51 μm深度处的双光子成像
Fig. 11. AO two-photon microscope system and comparison of images before and after AO correction[57]. (a) Schematic of AO two-photon microscope based on SHWS and open-loop control; (b) two-photon images of live Drosophila embryo at depth of 51 μm before and after AO correction
刘立新, 张美玲, 吴兆青, 杨乾乾, 郜鹏, 薛平. 自适应光学在荧光显微镜中的应用[J]. 激光与光电子学进展, 2020, 57(12): 120001. Lixin Liu, Meiling Zhang, Zhaoqing Wu, Qianqian Yang, Peng Gao, Ping Xue. Application of Adaptive Optics in Fluorescence Microscope[J]. Laser & Optoelectronics Progress, 2020, 57(12): 120001.
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