光学学报, 2018, 38 (4): 0430001, 网络出版: 2018-07-10 复制并引用该论文  本文已被引 3 次  被引通知

图 & 表

图 1. 3种浓度大肠杆菌悬浮液的透射光谱

Fig. 1. Transmission spectra of Escherichia coli suspension with three concentrations

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图 2. (a)光谱1颗粒浓度归一化后光密度的测量值与计算值;(b) 3条透射光谱的浓度拟合线

Fig. 2. (a) Measured and calculated optical density of spectrum 1 after particle concentration normalization; (b) concentration fitting curves of three different transmission spectra

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图 3. (a)光谱1最大值归一化后光密度的测量值与计算值; (b) 3条透射光谱的浓度拟合线

Fig. 3. (a) Measured and calculated optical density of spectrum 1 after maximum normalization; (b) concentration fitting curves of three different transmission spectra

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图 4. (a)光谱1积分归一化后光密度的测量值与计算值;(b) 3条透射光谱的浓度拟合线

Fig. 4. (a) Measured and calculated optical density of spectrum 1 after integral normalization; (b) concentration fitting curves of three different transmission spectra

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图 5. (a)光谱1平均归一化后光密度的测量值与计算值; (b) 3条透射光谱的浓度拟合线

Fig. 5. (a) Measured and calculated optical density of spectrum 1 after average normalization; (b) concentration fitting curves of three different transmission spectra

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表 1不同归一化透射光谱反演得到的大肠杆菌的结构参数

Table1. Structural parameters of Escherichia coli obtained with the inversion of different normalization transmission spectra

Spectrum Particle concentration normalization Maximum normalization Integral normalization Average normalization D1 /μm D2 /μm D1 /μm D2 /μm D1 /μm D2 /μm D1 /μm D2 /μm 1 1.4900 0.0500 1.7571 0.0501 1.2792 0.0501 1.2715 0.0501 2 1.3900 0.0500 1.4351 0.0501 1.4635 0.0501 1.4602 0.0501 3 1.4900 0.0500 1.4799 0.0501 1.4086 0.0525 1.4086 0.0500

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表 24种归一化法与平板计数法得到的水体大肠杆菌的浓度

Table2. Concentrations obtained with four normalization methods and serial dilution plate counting method

No. Concentration from plate counting /(107 mL-1) Particle concentration normalization Maximum normalization Integral normalization Average normalization Concentration/(107 mL-1) Error /% Concentration/(107 mL-1) Error /% Concentration/(107 mL-1) Error /% Concentration/(107 mL-1) Error /% 1 3.4200 2.3668 30.80 1.0008 70.74 3.3512 2.01 3.4300 0.29 2 2.5200 3.0712 21.87 2.7175 7.84 2.5248 0.19 2.5432 0.92 3 3.5000 2.7766 20.67 2.9230 16.49 3.5424 1.21 3.5310 0.89 Average relative error /% 24.45 31.69 1.14 0.70

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胡玉霞, 赵南京, 甘婷婷, 段静波, 孟德硕, 刘建国, 刘文清. 水体细菌微生物多波长透射光谱定量分析归一化方法研究[J]. 光学学报, 2018, 38(4): 0430001. Yuxia Hu, Nanjing Zhao, Tingting Gan, Jingbo Duan, Deshuo Meng, Jianguo Liu, Wenqing Liu. Normalization of Quantitative Analysis for Bacteria Multi-Wavelength Transmission Spectroscopy in Water[J]. Acta Optica Sinica, 2018, 38(4): 0430001.