3μs,200ns和2ns脉冲电场对酵母菌杀灭效果分析

研究了脉冲宽度对高压脉冲下细胞膜和细胞器膜跨膜电压的影响及细胞膜能量的变化,以及13 μs,200 ns和2 ns脉冲对酿酒酵母（CICC31180）杀灭作用时脉冲数的影响。结果表明,随着脉冲数增加,13 μs,200 ns和2 ns脉冲对啤酒酵母的灭杀效果均增强。在13 μs脉宽,14 kV电压和50个脉冲数下,酵母达到最小存活率-4.3个对数；在200 ns脉宽,40 kV电压和500个脉冲数下,酵母达到存活率-2.6个对数；在2 ns脉宽,200 kV电压和500个脉冲数下,酵母达到存活率-2.8个对数。三种脉冲对酵母菌杀灭的存活率对数与脉冲数的关系表现不是线性的,脉冲数增加到一定程度,存活率下降变缓。在同等灭杀效果-2.0个对数下,2 ns脉冲比13 μs脉冲在同轴电极中损耗的能量更少。

Abstract

The effect of the pulse width change is studied on the membrane voltage and energy of cell membranes and organelles membrane under the high voltage pulse electric field. The sterilization effect of saccharomyces cerevisiae (CICC31180) changes with the quantity of the 13 μs, 200 ns or 2 ns pulses. The results showed that with the increase of the quantity of pulses, the sterilization effect was enhanced. The yeast concentration decreased by 4.3 orders of magnitude after 50, 13 μs width, 14 kV pulses treatment while by 2.8 orders of magnitude after 500, 13 ns width 200 kV pulses treatment, by 2.6 orders of magnitude after 500, 13 ns width 200 kV pulses treatment. The survival rate logarithmic relation with the pulse number was not linear. The survival rate logarithmic decreased slowly when the pulse number increased to a higher value. Under the same survival rate logarithmic decrease, the loss of energy in coaxial electrode was lower in 2 ns pulses than 13 μs or 200 ns pulses.

参考文献

[1] 张若兵, 陈杰, 肖健夫, 等. 高压脉冲电场设备及其在食品非热处理中的应用［J］. 高电压技术, 2011, 37(3): 777-786.

Zhang Ruobing, Chen Jie, Xiao Jianfu, et al. Pulsed electric fields system and its application in non-thermal food processing. High Voltage Engineering, 2011, 37(3): 777-786

[2] 江伟华. 高重复频率脉冲功率技术及其应用：（6）代表性的应用［J］. 强激光与粒子束, 2014, 26: 030201.

Jiang Weihua. Repetition rate pulsed power technology and its applications: (ⅵ) typical applications. High Power Laser and Particle Beams, 2014, 26: 030201

[3] 陈新梅, 李莹, 陈新华. 高压脉冲电场灭菌方法研究概况［J］. 中国执业药师, 2014, 11(s1): 38-41.

Chen Xinmei, Li Ying, Chen Xinhua. Research on the high-voltage pulsed electric field sterilization method. China Licensed Pharmacist, 2014, 11(s1): 38-41

[4] 张悦, 李国锋, 李杰. 高压脉冲电场水中杀菌［J］. 河北大学学报：自然科学版, 2007, 27(s1): 78-81.

Zhang Yue, Li Guofeng, Li Jie. Water sterilization by pulsed electric field (PEF). Journal of Hebei University: Natural Science Edition, 2007, 27(s1): 78-81

[5] Schoenbach K H, Joshi R P, Kolb J F, et al. Ultrashort electrical pulses open a new gateway into biological cells［J］. Proceedings of the IEEE, 2004, 92(7): 1122-1137.

[6] Chen C, Smye S W, Robinson M P, et al. Membrane electroporation theories: a review［J］. Medical & Biological Engineering & Computing, 2006, 44(1): 5-14.

[7] Beebe S J, Fox P M, Rec L J, et al. Nanosecond pulsed electric field (nsPEF) effects on cells and tissues: apoptosis induction and tumor growth inhibition［J］. IEEE Trans Plasma Sci, 2001, 30(1): 286-292.

[8] 覃玉荣. 外电场作用下悬液中细胞膜电压变化建模研究［D］. 广州：华南理工大学, 2005: 46-47.

Qin Yurong. Research on modeling the transmembrane voltage induced on a cell membrane in suspensions exposed to external electric fields. Guangzhou: South China University of Technology, 2005: 46-47

[9] 方婷, 王仁丽, 严志明, 等. 20 kV/cm高压脉冲电场下外界因素对大肠杆菌杀灭效果的影响［J］. 贵州大学学报：自然科学版, 2007, 24(4): 431-435.

Fang Ting, Wang Renli, Yan Zhiming, et al. Effect of environmental factors on E. coli inactivation under 20 kV/cm PEF. Journal of Guizhou University: Natural Science Edition, 2007, 24(4): 431-435

[10] 廖小军, 钟葵, 王黎明, 等. 高压脉冲电场对酵母和大肠杆菌的杀灭效果［J］. 食品与发酵工业, 2003, 29(10): 19-22.

Liao Xiaojun, Zhong Kui, Wang Liming, et al. Inactivation of saccharomyces cerevisiae and E. coli by pulsed electric fields technology. Food and Fermentation Industries Editorial Staff, 2003, 29(10): 19-22

[11] 李亚维, 丁明军, 马成刚, 等. 80 kV可调节高压脉冲方波脉冲电源的研制［J］. 强激光与粒子束, 2013, 25(10): 2742-2746.

Li Yawei, Ding Mingjun, Ma Chenggang, et al. 80 kV adjustable high-voltage rectangle-wave pulsed power supply. High Power Laser and Particle Beams, 2013, 25(10): 2742-2746

[12] 马成刚, 李玺钦, 李亚维, 等. 150 kV快前沿低抖动Marx发生器研制［J］. 强激光与粒子束, 2015, 27: 045001.

Ma Chenggang, Li Xiqin, Li Yawei, et al. Development of 150 kV fast risetime low jitter Marx generator. High Power Laser and Particle Beams, 2015, 27: 045001

[13] 林荣华, 李伟光. 高压脉冲电场灭菌设备及其流量控制［J］. 机械制造与自动化, 2012, 41(2): 177-179.

Lin Ronghua, Li Weiguang. High-voltage pulsed electric field sterilization equipment and flow control. Machine Building & Automation, 2012, 41(2): 177-179

金雪剑, 李洪涛, 邓维军, 江艳, 何萍. 3μs,200ns和2ns脉冲电场对酵母菌杀灭效果分析[J]. 强激光与粒子束, 2015, 27(12): 125005. Jin Xuejian, Li Hongtao, Deng Weijun, Jiang Yan, He Ping. Analysis of inactivation saccharomyces cerevisiae by 13 μs, 200 ns and 2 ns pulsed electric fields[J]. High Power Laser and Particle Beams, 2015, 27(12): 125005.

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