系统回顾了衰减全反射傅里叶变换红外光谱（attenuated total reflection Fourier transform infrared, ATR-FTIR)技术在临床医学领域中的应用研究进展。 ATR-FTIR光谱技术具有实时、 简单、 无创扫描生物组织样品的优势, 通过结合多模式识别统计学方法并对照临床及病理学诊断结果, 可对生物组织样本进行判别分析。 目前, 对甲状腺、 乳腺, 以及肺组织等新鲜离体组织的光谱学检测中, ATR-FTIR技术可达到较高的判别敏感性、 准确性与特异性; 尤其在对甲状腺和乳腺疾病患者的前哨淋巴结活检的研究中, 应用ATR-FTIR技术, 可对良、 恶性组织进行敏感性、 准确性与特异性均较高的鉴别研究, 对辅助临床诊断具有潜在的重要价值。 此外, 利用ATR-FTIR光谱技术对生物体液进行光谱分析并进一步诊断疾病的研究也在逐步开展。 由于血清中所含成分的改变对于提示疾病状况具有重要价值, 因而血清ATR-FTIR光谱学诊断方法成为研究热点, 已有应用ATR-FTIR技术对脑胶质细胞瘤、 心肌梗死、 肾衰、 阿尔茨海默病、 卵巢癌与子宫内膜癌等多种疾病进行分析的研究报道。 目前已可通过ATR-FTIR技术对卵巢癌进行疾病分期的判别诊断, 该技术有望成为卵巢癌筛查的重要方法。 由于血清ATR-FTIR光谱学技术具有检测快速、 判别准确、 性价比高等优势, 有望成为今后生物医学发展的重要方向之一。

NAD+/NADH redox state has been implicated in many diseases such as cancer and diabetes as well as in the regulation of embryonic development and aging. To fluorimetrically assess the mitochondrial redox state, Dr. Chance and co-workers measured the fluorescence of NADH and oxidized flavoproteins (Fp) including flavin–adenine–dinucleotide (FAD) and demonstrated their ratio (i.e. the redox ratio) is a sensitive indicator of the mitochondrial redox states. The Chance redox scanner was built to simultaneously measure NADH and Fp in tissue at submillimeter scale in 3D using the freeze-trap protocol. This paper summarizes our recent research experience, development and new applications of the redox scanning technique in collaboration with Dr. Chance beginning in 2005. Dr. Chance initiated or actively involved in many of the projects during the last several years of his life. We advanced the redox scanning technique by measuring the nominal concentrations (in reference to the frozen solution standards) of the endogenous fluorescent analytes, i.e., [NADH] and [Fp] to quantify the redox ratios in various biological tissues. The advancement has enabled us to identify an array of the redox indices as quantitative imaging biomarkers (including [NADH], [Fp], [Fp]/([NADH]+[Fp]), [NADH]/[Fp], and their standard deviations) for studying some important biological questions on cancer and normal tissue metabolism. We found that the redox indices were associated or changed with (1) tumorigenesis (cancer versus non-cancer of human breast tissue biopsies); (2) tumor metastatic potential; (3) tumor glucose uptake; (4) tumor p53 status; (5) PI3K pathway activation in premalignant tissue; (6) therapeutic effects on tumors; (7) embryonic stem cell differentiation; (8) the heart under fasting. Together, our work demonstrated that the tissue redox indices obtained from the redox scanning technique may provide useful information about tissue metabolism and physiology status in normal and diseased tissues. The Chance redox scanner and other redox imaging techniques may have wide-ranging potential applications in many fields, such as cancer, diabetes, developmental process, mitochondrial diseases, neurodegenerative diseases, and aging.

系统回顾了过去50余年中红外光谱技术的发展创新及在生物医学领域中的研究应用。 傅里叶变换红外光谱技术已从对单个细胞水平结构构成与形态的研究进展到组织水平。现代红外光谱技术联合成熟的模式识别技术与组织微阵列技术,为平行大规模测定分子结构提供了可能。 此外,红外光谱技术的临床应用为良恶性肿瘤的判别提供了可信的参考依据,并可准确判别甲状腺、乳腺、胃肠道及腮腺等组织的良恶性,具有极广泛的应用前景。

用光动力学疗法治疗了皮肤恶性肿瘤35例。治疗中应用输出功率为600 mW的He-Ne激光器和血卟啉。随访3～9个月，近期痊愈率达91.43%(32例)，显效的为8.57%(3例)。