基于线性混合的光度法定量检测芝麻油掺杂

基于芝麻油和掺入油线性混合的原理，提出了一种利用芝麻油、掺入油和其掺混油的吸光度直接计算掺杂比的定量检测方法。利用误差分析理论对掺杂比计算公式进行分析，可知使误差最小的最佳检测波长区位于芝麻油和掺入油的吸光度差值最大的区域。利用SpectraMax Plus 384型光吸收酶标仪的分光光度计功能和构建的可见近红外光谱采集系统，对两种光程的芝麻油分别掺杂花生油和大豆油的样本进行了检测。对掺杂体积比为10%以上的芝麻花生掺混油和芝麻大豆掺混油，分光光度计检测的掺杂比计算值和实际值的相关系数分别为0.9992和0.9997，标准误差分别为0.0084和0.0054；可见近红外光谱采集系统检测的两种掺杂比的相关系数分别为0.9997和0.9994，标准误差分别为0.0047和0.0069。实验结果表明，利用线性光度法计算掺杂芝麻油的掺杂比例是准确可行的。

Abstract

Based on the linear mixing principle of sesame oil and adulterated oil, a photometric method for direct determination of the doping ratio is proposed by using the absorbance of sesame oil, adulterated oil and their mixed oil. The error analysis method is used to analyze the formula of doping ratio. It can be seen that the optimal detection wavelength region is the region where the absorbance difference between sesame oil and adulterated oil is the biggest. The spectrophotometer functions of the SpectraMax Plus 384 microplate reader and the assembled visible-near infrared spectral acquisition system are used to test the sesame oil doped with peanut oil and soybean oil. For the sesame oil with the doping volume ratio of peanut oil and soybean oil larger than 10%, the testing results of the spectrophotometer show that the correlation coefficients between the calculated values and the actual values of the doping ratio are 0.9992 and 0.9997, and the standard errors are 0.0084 and 0.0054, respectively. Meanwhile, the testing results of the visible-near infrared spectral acquisition system show that the correlation coefficients are 0.9997 and 0.9994, and the standard errors are 0.0047 and 0.0069, respectively. The experimental results indicate that the proposed linear photometric method for quantitative determination of doping components in sesame oil is feasible and accurate.

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许洪, 杨基春. 基于线性混合的光度法定量检测芝麻油掺杂[J]. 激光与光电子学进展, 2017, 54(3): 031201. Xu Hong, Yang Jichun. Quantitative Determination of Adulteration in Sesame Oil Based on Linear Photometry[J]. Laser & Optoelectronics Progress, 2017, 54(3): 031201.

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