Author Affiliations
Abstract
1 State Key Laboratory of Extreme Photonics and Instrumentation, College of Optical Science and Engineering, Zhejiang University, Hangzhou 310027, P. R. China
2 ZJU-Hangzhou Global Scientific and Technological Innovation Center, Hangzhou 311200, P. R. China
3 Collaborative Innovation Center of Extreme Optics, Shanxi University, Taiyuan, Shanxi 030006, P. R. China
4 Advanced Biomedical Imaging Facility-Wuhan National Laboratory for Optoelectronics, Huazhong University of Science and Technology, Wuhan, Hubei 430074, P. R. China
Structured illumination microscopy (SIM) achieves super-resolution (SR) by modulating the high-frequency information of the sample into the passband of the optical system and subsequent image reconstruction. The traditional Wiener-filtering-based reconstruction algorithm operates in the Fourier domain, it requires prior knowledge of the sinusoidal illumination patterns which makes the time-consuming procedure of parameter estimation to raw datasets necessary, besides, the parameter estimation is sensitive to noise or aberration-induced pattern distortion which leads to reconstruction artifacts. Here, we propose a spatial-domain image reconstruction method that does not require parameter estimation but calculates patterns from raw datasets, and a reconstructed image can be obtained just by calculating the spatial covariance of differential calculated patterns and differential filtered datasets (the notch filtering operation is performed to the raw datasets for attenuating and compensating the optical transfer function (OTF)). Experiments on reconstructing raw datasets including nonbiological, biological, and simulated samples demonstrate that our method has SR capability, high reconstruction speed, and high robustness to aberration and noise.
Structured illumination microscopy image reconstruction spatial domain digital micromirror device (DMD) Journal of Innovative Optical Health Sciences
2024, 17(2): 2350021
1 中国科学院西安光学精密机械研究所瞬态光学与光子技术国家重点实验室,陕西 西安 710119
2 中国科学院大学,北京 100049
将普通光学显微镜的均匀照明替换为光场具有空间结构分布的照明,可为显微镜增添超分辨和光切片的新功能。结构光照明显微(SIM)技术与传统宽场光学显微镜具有良好的结构兼容性,继承了传统光学显微镜非侵入、低光毒性、低荧光漂白、快速成像的优点。其高时空分辨率和三维光切片能力非常适合活体细胞或组织的观测,受到生物医学和光学界的持续关注。快速产生高对比度、高频率的结构光场并进行快速相移和旋转调控是SIM的核心技术。近年来基于数字微镜器件(DMD)调制的SIM(DMD-SIM)发展迅速,它利用DMD高刷新率、高光通量、偏振不敏感的优势,克服了传统器件如物理光栅和液晶空间光调制器在调控速度上的缺点。本综述首先介绍了SIM超分辨和光切片的基本原理,然后着重阐述了DMD-SIM通过光投影和光干涉产生结构光照明及调控光场的方法,对当前的DMD-SIM研究进展进行了归纳评述,总结了DMD-SIM的优缺点,最后对DMD-SIM面临的挑战和发展趋势进行了展望。
光学显微 结构光照明显微 超分辨 光切片 数字微镜器件 激光与光电子学进展
2024, 61(6): 0618001
Author Affiliations
Abstract
1 International Collaborative Laboratory of 2D Materials for Optoelectronics Science and Technology of Ministry of Education, Institute of Microscale Optoelectronics, Shenzhen University, Shenzhen, China
2 Institute for Advanced Study, Shenzhen University, Shenzhen, China
3 Fiber Optics Research Centre, School of Information and Communication Engineering, University of Electronic Science and Technology of China, Chengdu, China
4 Departamento de Física, Universidade Federal de Pernambuco, Recife-PE, Brazil
5 Email: hejingsong@szu.edu.cn
High-intensity vortex beams with tunable topological charges and low coherence are highly demanded in applications such as inertial confinement fusion (ICF) and optical communication. However, traditional optical vortices featuring nonuniform intensity distributions are dramatically restricted in application scenarios that require a high-intensity vortex beam owing to their ineffective amplification resulting from the intensity-dependent nonlinear effect. Here, a low-coherence perfect vortex beam (PVB) with a topological charge as high as 140 is realized based on the super-pixel wavefront-shaping technique. More importantly, a globally adaptive feedback algorithm (GAFA) is proposed to efficiently suppress the original intensity fluctuation and achieve a flat-top PVB with dramatically reduced beam speckle contrast. The GAFA-based flat-top PVB generation method can pave the way for high-intensity vortex beam generation, which is crucial for potential applications in ICF, laser processing, optical communication and optical trapping.
digital micromirror device flat-top beam orbital angular momentum perfect vortex beam random fiber laser High Power Laser Science and Engineering
2024, 12(1): 010000e5
Author Affiliations
Abstract
1 Peking University, College of Future Technology, Department of Biomedical Engineering, Beijing, China
2 Peking University, National Biomedical Imaging Center, Beijing, China
In recent years, notable progress has been achieved in both the hardware and algorithms of structured illumination microscopy (SIM). Nevertheless, the advancement of three-dimensional structured illumination microscopy (3DSIM) has been impeded by challenges arising from the speed and intricacy of polarization modulation. We introduce a high-speed modulation 3DSIM system, leveraging the polarization-maintaining and modulation capabilities of a digital micromirror device (DMD) in conjunction with an electro-optic modulator. The DMD-3DSIM system yields a twofold enhancement in both lateral (133 nm) and axial (300 nm) resolution compared to wide-field imaging and can acquire a data set comprising 29 sections of 1024 pixels × 1024 pixels, with 15 ms exposure time and 6.75 s per volume. The versatility of the DMD-3DSIM approach was exemplified through the imaging of various specimens, including fluorescent beads, nuclear pores, microtubules, actin filaments, and mitochondria within cells, as well as plant and animal tissues. Notably, polarized 3DSIM elucidated the orientation of actin filaments. Furthermore, the implementation of diverse deconvolution algorithms further enhances 3D resolution. The DMD-based 3DSIM system presents a rapid and reliable methodology for investigating biomedical phenomena, boasting capabilities encompassing 3D superresolution, fast temporal resolution, and polarization imaging.
digital micromirror device electro-optic modulation polarization three-dimensional structured illumination microscopy Advanced Photonics Nexus
2024, 3(1): 016001
光学 精密工程
2023, 31(21): 3096
1 重庆邮电大学通信与信息工程学院,重庆 400065
2 移动通信教育部工程研究中心,重庆 400065
为了提高纠缠光量子成像效率,本文采用双步符合计数法快速获取目标成像信息,降低纠缠光量子成像的时间开销。首先,利用透镜和波片组合对激光器产生的泵浦光进行调制,提高周期极化磷酸氧钛钾(PPKTP)晶体自发参量下转换的效率;然后,通过数字微镜器件(DMD)选取测距区域,构造单光子时间脉冲序列差值;其次,利用该差值完成局部符合计数以得到信号和参考光路的延时差;再次,通过控制DMD选取成像区域,对单光子时间脉冲序列进行修正,并利用修正后的序列完成全局符合计数;最后,将符合计数值与灰度值进行映射,得到目标的量子图像。此外,通过与经典量子成像结果相比较,分析了目标距离、测距区域大小和单像素曝光时间对成像结果的影响,同时搭建了实际的量子成像光路以验证本文方法的有效性。
量子纠缠 量子成像 符合计数 数字微镜器件 成像效率 光学学报
2023, 43(20): 2027003
1 中国科学院上海技术物理研究所, 上海 200083
2 上海科技大学 信息科学与技术学院, 上海 200120
作为常用显示器件,数字微镜器件(DMD)使用传统的脉冲宽度调制(PWM)显示方法受最小脉冲宽度限制,无法满足高帧频显示的需求。文章提出基于光源与DMD复合编码的高帧频显示技术,利用光源调制解决脉冲宽度调制导致的位平面显示时间随位平面等级指数增长的问题。通过构建包含驱动模块、光源和DMD的显示系统,采用低4位光源脉冲宽度调制与高4位DMD显示时间宽度调制相结合的方法,将8位灰度图像的显示帧频提高至2461Hz。
数字微镜器件 高帧频 复合编码 光源调制 脉冲宽度调制 digital micromirror device high frame frequency composite coding illuminant modulation pulse width modulation
安徽工程大学机械工程学院,安徽 芜湖 241000
无掩模光刻技术具有无需物理掩模、成本低、适合大批量生产的优点,在微结构制作中得到了广泛应用。基于数字微镜器件(DMD)的无掩模数字光刻技术具有分辨率高、灵活性好、加工精度高等优势,成为近年来数字光刻领域的研究热点。综述了DMD数字光刻技术的研究进展,包括基于DMD的扫描光刻技术、步进式光刻技术以及灰阶光刻技术,介绍了该方法在集成电路、微光学、三维打印等领域的应用,并总结了目前DMD光刻技术存在的问题及其未来发展趋势。
光学设计 无掩模 数字微镜器件 扫描光刻技术 步进式光刻技术 灰阶光刻技术 激光与光电子学进展
2022, 59(11): 1100010