基于表面增强拉曼光谱技术的豆芽6-BA残留快速检测方法

采用快速溶剂提取前处理技术与表面增强拉曼光谱（surface-enhanced Raman spectroscopy, SERS）检测技术建立豆芽中6-苄基腺嘌呤（6-benzylaminopurine, 6-BA）残留物的快速定量检测方法。以自发豆芽为空白对照, 通过对梯度浓度的6-BA插标豆芽提取液进行SERS检测发现, 在1 002 cm-1处具有明显的6-BA特征拉曼峰, 且归一化处理后的特征峰峰强与6-BA浓度呈良好的线性关系, 高浓度与低浓度的线性范围分别为0.5～14 μg·mL-1与0.1～2 μg·mL-1, 最低定量检出限为0.02 mg·kg-1, 回收率为82.3%～95.1%, 相对标准偏差小于5%。该方法重现性较好、前处理简单、全程检测耗时短、设备操作简便, 为市场大规模的快速检测提供了现场、高效、灵敏的解决方案。

Abstract

New method for rapid and quantitative analysis of 6-benzylaminopurine (6-BA) residues in sprout beans was studied by using FAST-SPE and portable surface-enhanced Raman spectroscopy (SERS). With homemade sprout bean samples as blank control, 6-BA solutions extracted from inserted-treatment samples showed typical characteristic Raman peaks at 1 002 cm-1 tested by SERS, and normalized 1 002 cm-1 intensities had a good linear relationship with gradient concentrations of 6-BA insert-standard solutions. The high concentration linear range was 0.5～14 μg·mL-1, and the low one was 0.1～2 μg·mL-1. The quantitative detection limit was down to 0.02 mg·kg-1 that was below the maximum allowable residues (MAL) of 0.2 mg·kg-1 in related National Standard. The recoveries were 82.3%～95.1% and the RSD was below 5%. This method with portable equipment and simple pre-treatment showed good reproducibility, short test time-consuming and easy operating, and thus it would provide efficient and sensitive solutions for large-scale on-site and rapid detections.

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张萍, 郑大威, 刘晶, 钟儒刚, 刘春伟, 郭浔, 汪泓. 基于表面增强拉曼光谱技术的豆芽6-BA残留快速检测方法[J]. 光谱学与光谱分析, 2012, 32(5): 1266. ZHANG Ping, ZHENG Da-wei, LIU Jing, ZHONG Ru-gang, LIU Chun-wei, GUO Xun, WANG Hong. Rapid Detection of 6-Benzylaminopurine Residues in Sprout Beans by Surface-Enhanced Raman Spectroscopy[J]. Spectroscopy and Spectral Analysis, 2012, 32(5): 1266.

基于表面增强拉曼光谱技术的豆芽6-BA残留快速检测方法

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