We develop optical fiber nanoprobe by spark fused taper and acid corrosion methods. By coupling with 3-aminopropyltrimethoxysilane, gold nanoparticles are solidified onto the surface of fiber optic and then the optical fiber sensor is prepared using surface-enhanced Raman spectroscopy (SERS) measurement of the cell solution. The SERS of the esophagus cancer cell solution is then measured by direct detection and fiber detection methods, and the relationship between SERS fiber detection and the length of optical fiber ensor is studied. This is helpful for the SERS measurement of tissues and organs using the optical fiber sensor.

Multi-reference configuration interaction is used to produce potential energy curves (PECs) for the excited B1\Pi state of KH molecule. To investigate the correlation effect of core-valence electrons, five schemes are employed which include the different correlated electrons and different active spaces. The PECs are fitted into analytical potential energy functions (APEFs). The spectroscopic parameters, ro-vibrational levels, and transition frequencies are determined based on the APEFs and compared with available experimental and theoretical data. The molecular properties for B1| obtained in this letter, which are better than those available in literature, can be reproduced with calculations using the suitable correlated electrons and active space of orbitals.

A frequency-stabilized 556-nm laser is an essential tool for experimental studies associated with 1S0-3P1 intercombination transition of ytterbium (Yb) atoms. A 556-nm laser light using a single-pass second harmonic generation (SHG) is obtained in a periodically poled MgO:LiNbO3 (PPLN) crystal pumped by a fiber laser at 1111.6 nm. A robust frequency stabilization method which facilitates the control of laser frequency with an accuracy better than the natural linewidth (187 kHz) of the intercombination line is developed. The short-term frequency jitter is reduced to less than 100 kHz by locking the laser to a home-made reference cavity. A slow frequency drift is sensed by the 556-nm fluorescence signal of an Yb atomic beam excited by one probe beam and is reduced to less than 50-kHz by a computer-controlled servo system. The laser can be stably locked for more than 5 h. This frequency stabilization method can be extended to other alkaline-earth-like atoms with similar weak intercombination lines.

The action mode between Dy(III)(NR)₃ and herring sperm DNA is studied by ultraviolet-visible (UV-vis) and fluorescence spectra as well as electrochemistry. Double-reciprocal method studies show that the binding stoichiometry between Dy(III)(NR)₃ and DNA is 1:1, the binding constants at different temperatures are 1。15×10⁵ L/mol at 25 ℃ and 2.09×10⁵ L/mol at 35 ℃, and the corresponding thermodynamic parameters are \Delta_rH \mathrm m = 2.48×10⁴ J/mol, \Delta_rG \mathrm m =-2.34×10⁴ J/mol, \Delta_rSm=161.7 J/(mol·K); \Delta_rS \mathrm m is the driving force in this reaction. Combined with Scatchard method and melting method, the results suggest that the interaction mode between Dy(III)(NR)₃ and herring sperm DNA is intercalation fashion and groove fashion.

The potential energy curves (PECs) of BO molecule, including \sum+ and \prod symmetries with doublet spin multiplicities, are obtained employing multi-reference configuration interaction (MRCI) method and Dunning’s correlation consistent basis sets. The analytical potential energy functions (APEFs) are fitted using the Murrell-Sorbie (MS) function and the least square method. Based on the PECs, the spectroscopic constants of the states have been determined and compared with the theoretical and experimental results available to affirm the accuracy and liability of the calculations. The root-mean-square (RMS) errors between the fitted results and the ab initio values are too little in comparison with the chemical accuracy (349.755 cm-1. It is shown that the present APEFs are accurate and can display the interaction between the atoms well. The present APEFs can be used to construct more complicated APEF or do some dynamic investigations.

Fluorescence spectra of acetic acid-water solution excited by ultraviolet (UV) light are studied, and the relationship between fluorescence spectra and molecular association of acetic acid is discussed. The results indicate that when the exciting light wavelength is longer than 246 nm, there are two fluorescence peaks located at 305 and 334 nm, respectively. By measuring the excitation spectra, the optimal wavelengths of the two fluorescence peaks are obtained, which are 258 and 284 nm, respectively. Fluorescence spectra of acetic acid-water solution change with concentrations, which is primarily attributed to changes of molecular association of acetic acid in aqueous solution. Through theoretical analysis, three variations of molecular association have been obtained in acetic acid-water solution, which are the hydrated monomers, the linear dimers, and the water separated dimers. This research can provide references to studies of molecular association of acetic acid-water, especially studies of hydrogen bonds.

The ultraviolet (UV) light excited fluorescence spectra of ethanol-water mixture with different concentrations are investigated by derivative fluorimetry. It is found that there are 8 types of luminescent cluster molecules, formed by ethanol and water molecules in different ways, existing in the solution. The peak wavelengths of all these clusters’ fluorescence spectra are measured and their contents are obtained by measuring the peak values in the second derivative fluorescence spectra. The spectra corresponding to the 8 types of clusters are obtained by Gaussian decomposition. It is found that two kinds of cluster molecules whose peak wavelengths are 330 and 345 nm have an optimal excitation wavelength located at (236+-3) nm. This research contributes to the study of ethanol-water cluster structures and their physical and chemical characteristics.

A feasible method of combining the concept of fluorescence half-life and the power dependent photobleaching rate for characterizing the practical photostability of fluorescent proteins (FPs) was introduced. Furthermore, by using a fluorescent photostability standard, a relative comparison of the photostabilty of FPs from different research groups was proposed, which would be of great benefit for developing novel FPs with optimized emission wavelength, better brightness, and improved photostability. We used rhodamine B as an example to verify this method and evaluate the practical photostability of a far-red FP, mKate-S158C. Experimental results indicated good potential of this method for further study.